GeneTex

  • Country / Location Selection

United States (US)

Rabbit Anti-Mouse lamda Light Chain antibody [RM110]

Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857
Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857
Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857

Cat. No. GTX60857

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM110

Isotype

 IgG

Application

 WB, FACS, IP, ELISA

Reactivity

 Mouse
Package
100 μg ($369)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1μg/mL - 0.5 μg/mL
FACS Assay dependent
IP Assay dependent
ELISA 0.005 μg/mL - 0.2 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The Fc region of RM110 has been engineered to eliminate Fc receptor binding.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgM lambda

Target Isotype

IgG (lambda light chain)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. This locus has four J-C clusters and three V segments that constitute two transcriptional units. One of the J-C clusters is incapable of encoding a protein; the J and C gene segments in this cluster are considered pseudogenes. Inbred strains of laboratory mice lack an additional J-C cluster of pseudogenes and several additional V segments that are found in wild mice. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 WB Image

WB analysis of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane) using GTX60857 Rabbit Anti-Mouse lamda Light Chain antibody [RM110].
Dilution : 0.2μg/ml

Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60857 Rabbit Anti-Mouse lamda Light Chain antibody [RM110].

Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. GTX60857

GTX60857 ELISA Image

ELISA analysis of mouse immunoglobulins (50 ng/well) using GTX60857 Rabbit Anti-Mouse lamda Light Chain antibody [RM110]. No cross reactivity with the kappa lightchain, human IgG, rat IgG, or goat IgG.
Dilution : 200 ng/mL, 50 ng/mL, or 10 ng/mL ofRM110 was used as the primary antibody.

REFERENCE

There are currently no references for Rabbit Anti-Mouse lamda Light Chain antibody [RM110] (GTX60857). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Rabbit Anti-Mouse lamda Light Chain antibody [RM110] (GTX60857). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 369