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Rabbit CXCL8 / IL8 protein (active)

Rabbit CXCL8 / IL8 protein (active). GTX00339-pro
Rabbit CXCL8 / IL8 protein (active). GTX00339-pro
Rabbit CXCL8 / IL8 protein (active). GTX00339-pro

Cat No. GTX00339-pro

Application Functional Assay
Species Rabbit
Package
10 μg ($319)
APPLICATION

Application Note

Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of IL8 on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100 μl cell suspension, 10 6 cells/ml in RPMI-1640 with FBS free) and IL8 (0. 1 ng/ml, 1 ng/ml and 10 ng/ml diluted separately in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). The migrated Jurkat cells in low chamber at low magnification (×100) randomly, and the migrated cells were counted at high magnification (×400). IL8 is able to induce migration of Jurkat cells, and the optimum chemotaxis of IL8 occurs at 1~10 ng/ml.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala23~Ser101 (NP_001075762.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Rabbit CXCL8 / IL8 protein (active). GTX00339-pro

GTX00339-pro Functional Assay Image

The chemotactic effect of GTX00339-pro Rabbit CXCL8 / IL8 protein (active).
Upper: (A) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 without IL8 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
(B) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 with 1 ng/ml IL8 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1hr.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Rabbit CXCL8 / IL8 protein (active). GTX00339-pro

GTX00339-pro Image

SDS-PAGE analysis of GTX00339-pro Rabbit CXCL8 / IL8 protein (active).

Rabbit CXCL8 / IL8 protein (active). GTX00339-pro

GTX00339-pro Image

WB analysis of GTX00339-pro Rabbit CXCL8 / IL8 protein (active).

REFERENCE
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