GeneTex
United States (US)

Rabbit IgG antibody (DyLight594)

GTX213110-05_20150814_IHC-Fr_M_18121410_614.jpg
GTX213110-05_42046_20180227_FACS_18121410_757.jpg
GTX213110-05_43510_20190306_ICC_IF_19042202_598.jpg

Cat No. GTX213110-05

Host Goat
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity Human, Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

100mM phosphate buffer (pH9.0)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Antigen Species

Rabbit

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight594

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Goat Anti Rabbit IgG
DATA IMAGES
GTX213110-05_20150814_IHC-Fr_M_18121410_614.jpg

GTX213110-05 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).
Blue: Nuclear staining with Hoechst 33342.

GTX213110-05_42046_20180227_FACS_18121410_757.jpg

GTX213110-05 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4°C for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
The Rabbit IgG antibody (DyLight594) (GTX213110-05) was used to detect the primary antibody.

GTX213110-05_43510_20190306_ICC_IF_19042202_598.jpg

GTX213110-05 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
The signal was developed using Rabbit IgG antibody (DyLight594) (GTX213110-05) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 149