GeneTex
United States (US)

Rabbit IgG antibody, pre-adsorbed (HRP)

Rabbit-IgG-antibody-pre-adsorbed-HRP_Dot_GTX27090-1_18121411_371.jpg
Rabbit-IgG-antibody-pre-adsorbed-HRP_WB_GTX27090-1_18121411_286.jpg

Cat No. GTX27090

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, Dot, ELISA, IHC
Reactivity Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000 - 1:10,000
Dot Assay dependent
ELISA 1:20,000 - 1:100,000
IHC 1:1,000 - 1:5,000
Not tested in other applications.

Specificity/Sensitivity

This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 , 10 mg/mL Bovine Serum Albumin (BSA) , 0.01% (w/v) Gentamicin Sulfate.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit IgG whole molecule

Target Isotype

IgG

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Rabbit-IgG-antibody-pre-adsorbed-HRP_Dot_GTX27090-1_18121411_371.jpg

GTX27090 Dot Image

GeneTex secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein 250ng starting total load Co-incubated with GeneTex HRP conjugated Goat anti Rabbit IgG antibody (GTX27090) and Dylight 649 conjugated goat anti Rabbit IgG antiody at 1:20K in blocking buffer.

Rabbit-IgG-antibody-pre-adsorbed-HRP_WB_GTX27090-1_18121411_286.jpg

GTX27090 WB Image

GTX27090 secondary antibody was used at 1:40,000 in blocking buffer to detect a rabbit primary antibody by Western Blot. Anti p27 antibody (GTX48799, 1:1000 RT 30 minutes) showed detection of 0.1 µg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose and blocked with 1% BSA-TTBS overnight at 4°C. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Package List Price ($)
$ 129