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Rabbit IgG isotype control

Cat No. GTX35035

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Control, IHC
Package
100 μg ($79)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

100 mM borate buffered saline, pH 8.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Isotype control for Rabbit IgG , Rabbit IgG negative control , Polyclonal Rabbit IgG isotype control , Rabbit negative control for IgG , Rabbit isotype control for IgG , Rabbit IgG isotype control , Negative control for Rabbit IgG , Isotype control to Rab

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

REVIEW

Rabbit IgG isotype control Cat No. GTX35035

Rating ( Average 5 based on 3 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 29095915
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : FACS
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2018.PMID: 30042348
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal Reprod Biol Endocrinol in 2013.PMID: 23849271
Application Reference
Package List Price ($)
$ 79