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Rabbit IgG isotype control

Cat. No. GTX35035

Reference 56
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Control, IHC
Package
100 μg ($119)
See all Rabbit IgG isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

100mM Borate buffered saline pH8.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control for Rabbit IgG , Rabbit IgG negative control , Polyclonal Rabbit IgG isotype control , Rabbit negative control for IgG , Rabbit isotype control for IgG , Rabbit IgG isotype control , Negative control for Rabbit IgG , Isotype control to Rab

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

TTT Phan et al. Cell Death Discov 2023; 9 (1) : 340 Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress
AJ Little et al. JCI Insight 2023; : e166076 HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease
Saito K et al. J Biol Chem 2023; 299 (7) : 104890 Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activity.
Peng C et al. Cell Death Dis 2023; 14 (2) : 155 MLKL signaling regulates macrophage polarization in acute pancreatitis through CXCL10.
Application : Control
Lee SH et al. PLoS Pathog 2023; 19 (1) : e1011078 Characterization of a new CCCTC-binding factor binding site as a dual regulator of Epstein-Barr virus latent infection.
Application : ChIP assay
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Submit a Reference

REVIEW

Rabbit IgG isotype control Cat. No. GTX35035

Rating
( Average 5 based on 3 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Rabbit IgG isotype control. GTX35035
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29095915
Rabbit IgG isotype control. GTX35035
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : FACS
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2018. PMID: 30042348
Rabbit IgG isotype control. GTX35035
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal Reprod Biol Endocrinol in 2013. PMID: 23849271
Submit a Review
Application Reference
TTT Phan et al. Cell Death Discov 2023; 9 (1):340 Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress
AJ Little et al. JCI Insight 2023;:e166076 HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease
Saito K et al. J Biol Chem 2023; 299 (7):104890 Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activity.
Peng C et al. Cell Death Dis 2023; 14 (2):155 MLKL signaling regulates macrophage polarization in acute pancreatitis through CXCL10.
Application : Control
Lee SH et al. PLoS Pathog 2023; 19 (1):e1011078 Characterization of a new CCCTC-binding factor binding site as a dual regulator of Epstein-Barr virus latent infection.
Application : ChIP assay
Miao CC et al. Autophagy 2022; 18 (4):921-934 LC3A-mediated autophagy regulates lung cancer cell plasticity.
Application : ChIP assay
Barreto-Duran E et al. Front Immunol 2022; 13:991991 The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection.
Zhu S et al. Mol Cell Biol 2022; 42 (6):e0018821 CTCF-Induced lncRNA C5orf66-AS1 Facilitates the Progression of Triple-Negative Breast Cancer via Sponging miR-149-5p to Up-Regulate CTCF and CTNNB1 to Activate Wnt/β-Catenin Pathway.
Application : IP
Yamazaki S et al. Mucosal Immunol 2022; 15 (6):1321-1337 IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies.
Application : ChIP assay
Panpan Dong et al. Exp Ther Med 2022; 23 (2):164 Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 ?M bisphenol A exposure via human neural stem cells.
Application : ChIP assay
Wang YT et al. Cell Rep 2022; 38 (8):110354 K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.
Hildur Arnardottir et al. J Clin Invest 2021; 131 (24) The resolvin D1 receptor GPR32 transduces inflammation resolution and atheroprotection.
Application : FACS
Zou Q et al. iScience 2021; 24 (12):103396 Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.
Khanh VC et al. Stem Cells Dev 2021; 30 (15):758-772 Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles Reduce SARS-CoV2-Induced Inflammatory Cytokines Under High Glucose and Uremic Toxin Conditions.
Lee J et al. Int J Mol Sci 2021; 22 (2) Inhibition of Hippo Signaling Improves Skin Lesions in a Rosacea-Like Mouse Model.
Application : IHC-P
Chiu YF et al. Sci Rep 2020; 10 (1):8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Filandrov? R et al. Structure 2020; (Epub) Motif orientation matters: structural characterization of TEAD1 recognition of genomic DNA.
Ma J et al. Nat Commun 2020; 11 (1):1769 Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.
Sadek J et al. Nat Commun 2020; 11 (1):1786 Modulation of virus-induced NF-庥B signaling by NEMO coiled coil mimics.
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy.
Huang CC et al. Sci Rep 2019; 9 (1):19839 Differential IL-17A response to S. pneumoniae in adenoid tissue of children with sleep disordered breathing and otitis media with effusion.
Lara-Reyna S et al. Front Immunol 2019; 10:1789 Metabolic Reprograming of Cystic Fibrosis Macrophages via the IRE1帢 Arm of the Unfolded Protein Response Results in Exacerbated Inflammation.
Tang K et al. Oncogene 2019; (Epub) Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.
Inoue T et al. PLoS One 2019; 14 (6):e0218090 Upregulation of sphingosine-1-phosphate receptor 3 on fibroblast-like synoviocytes is associated with the development of collagen-induced arthritis via increased interleukin-6 production.
Katagiri T et al. Mucosal Immunol 2019; (Epub) JunB plays a crucial role in development of regulatory T cells by promoting IL-2 signaling.
Li C et al. Front Oncol 2019; 9:225 BAG3 Suppresses Loading of Ago2 to IL6 mRNA in Pancreatic Ductal Adenocarcinoma.
Koh KH et al. J Clin Invest 2019; (Epub) Non-immune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist.
Ma C et al. Onco Targets Ther 2019; 12:1475-1495 Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.
Morio K et al. Biomed Rep 2018; 8 (1):47-50 Expression of doublecortin and CaM kinase-like-1 protein in serrated neoplasia of the colorectum.
Fan SM et al. Biomaterials 2018; 167:121-131 Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.
Trezise S et al. Int J Mol Sci 2018; 19 (8) Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins.
Hagenau F et al. Am J Ophthalmol 2018; (Epub) Vitrectomy for diabetic macular edema: optical coherence tomography criteria and pathology of the vitreomacular interface.
Tanaka K et al. Growth Horm IGF Res 2018; 41:34-41 synergistic effects of tumor necrosis factor-α and *-like growth factor-i on survival of human trophoblast-derived bewo cell line.
Van Nieuwenhove E et al. J Allergy Clin Immunol 2018; (Epub) A kindred with Mutant IKAROS and autoimmunity.
Fan S. MY et al. Biomaterials 2018; 167:121-131 Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.
Chen YJ et al. Sci Rep 2017; 7 (1):3842 SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Krendl C et al. Proc Natl Acad Sci U S A 2017; 114 (45):E9579-E9588 GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency.
Lee J et al. PLoS One 2017; 12 (11):e0187189 Interaction of IL-6 and TNF-α contributes to endothelial dysfunction in type 2 diabetic mouse hearts.
Application : ICC/IF
Morio K et al. Biomedical Reports 2017; (Epub) Expression of doublecortin and CaM kinase-like-1 protein in serrated neoplasia of the colorectum.
Wang XH et al. Mol Med Rep 2017; (Epub) IL-33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L.
Hosoda K et al. Oncology Letters 2017; (Epub) Expression of methylation-modulated tumor-related genes in endoscopically resected early esophageal squamous neoplasia.
Application : IHC-P
Morel AP et al. Nat Med 2017; (Epub) A stemness-related ZEB1-MSRB3 axis governs cellular pliancy and breast cancer genome stability.
Application : IP
Hosoda K et al. Oncol Lett 2017; 14 (1):737-742 Expression of methylation-modulated tumor-related genes in endoscopically resected early esophageal squamous neoplasia.
Application : IHC-P
Li H et al. Oncol Lett 2016; 12 (5):3492-3498 Clinical implication of Tiam1 overexpression in the prognosis of patients with serous ovarian carcinoma.
Li H et al. Oncol Lett 2016; 12:3492-3498 Clinical implication of Tiam1 overexpression in the prognosis of patients with serous ovarian carcinoma.
Boneschansker L et al. J Immunol 2016; 197 (4):1389-98 Netrin-1 Augments Chemokinesis in CD4+ T Cells In Vitro and Elicits a Proinflammatory Response In Vivo.
Application : FACS
Chen HR et al. PLoS Negl Trop Dis 2016; 10 (7):e0004828 Dengue Virus Nonstructural Protein 1 Induces Vascular Leakage through Macrophage Migration Inhibitory Factor and Autophagy.
Kobayashi K et al. Otolaryngol Head Neck Surg 2015; 153 (2):218-24 Nerve Growth Factor Signals as Possible Pathogenic Biomarkers for Perineural Invasion in Adenoid Cystic Carcinoma.
Application : IHC
Wang H et al. Clin Exp Immunol 2015; Neutrophil extracellular traps can activate alternative complement pathways.
Application : ICC/IF, ELISA
Tran QK et al. J Biol Chem 2015; 290 (21):13293-307 Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b.
Application : WB
Beppu LY et al. J Allergy Clin Immunol 2014; 134 (5):1100-1107.e4 TGF-β1-induced phospholamban expression alters esophageal smooth muscle cell contraction in patients with eosinophilic esophagitis.
Application : FACS
Yang CC et al. PLoS One 2014; 9 (10):e106683 Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the cellular NXF1-p15 machinery.
Schittenhelm J et al. Int J Clin Exp Pathol 2013; 6 (12):2719-32 Comparing the expression of integrins αvβ3, αvβ5, αvβ6, αvβ8, fibronectin and fibrinogen in human brain metastases and their corresponding primary tumors.
Jandl T et al. Nucl Med Biol 2013; 40 (2):177-81 Melanoma stem cells in experimental melanoma are killed by radioimmunotherapy.
Application : FACS
Hayashi KG et al. Reprod Biol Endocrinol 2013; 11:62 Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta.
Application : Control, IHC
Hayashi KG et al. Anim Reprod Sci 2012; 134 (43163):150-7 Placental expression and localization of endothelin-1 system and nitric oxide synthases during bovine pregnancy.
Application : IHC
Package List Price ($)
$ 119