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Rabbit IgG isotype control

Cat No. GTX76672

Reference 2

Host

 Rabbit

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Blocking, IHC
Package
10 mg ($149)
See all Rabbit IgG isotype control products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Blocking Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute to a minimum of 1 mg/ml with a saline (150 mM NaCl) solution.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Purification

Fractionation and ion-exchange chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

REFERENCE

Application Reference

Tseng IC et al. J Pathol 2017; (Epub) Microinsertions in PRKACA cause activation of the protein kinase A pathway in cardiac myxoma.
Application : IHC-P
Tetsuka M et al. Reproduction 2016; 151 (1) : 73-82 Glucocorticoid metabolism in the bovine cumulus-oocyte complex matured in vitro.
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Package List Price ($)
$ 149