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Rabphilin 3A antibody

Anti-Rabphilin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX115646
Anti-Rabphilin 3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115646
Anti-Rabphilin 3A antibody used in Western Blot (WB). GTX115646
Anti-Rabphilin 3A antibody used in Western Blot (WB). GTX115646
Anti-Rabphilin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX115646

Cat. No. GTX115646

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rabphilin 3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Rabphilin 3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885388

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rabphilin 3A

Cellular Localization

Cell junction , synapse

Background

Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Rabphilin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX115646

GTX115646 IHC-P Image

Rabphilin 3A antibody detects Rabphilin 3A protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Rabphilin 3A antibody (GTX115646) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rabphilin 3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115646

GTX115646 ICC/IF Image

Rabphilin 3A antibody detects Rabphilin 3A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rabphilin 3A stained by Rabphilin 3A antibody (GTX115646) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rabphilin 3A antibody used in Western Blot (WB). GTX115646

GTX115646 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rabphilin 3A antibody (GTX115646) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rabphilin 3A antibody used in Western Blot (WB). GTX115646

GTX115646 WB Image

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Rabphilin 3A antibody (GTX115646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rabphilin 3A antibody used in IHC (Paraffin sections) (IHC-P). GTX115646

GTX115646 IHC-P Image

Rabphilin 3A antibody detects Rabphilin 3A protein at membrane on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
Rabphilin 3A antibody (GTX115646) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169