Rabphilin 3A antibody detects Rabphilin 3A protein at membrane on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
Rabphilin 3A antibody (GTX115646) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Rabphilin 3A antibody (GTX115646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rabphilin 3A antibody (GTX115646) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rabphilin 3A antibody detects Rabphilin 3A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rabphilin 3A stained by Rabphilin 3A antibody (GTX115646) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Rabphilin 3A antibody detects Rabphilin 3A protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Rabphilin 3A antibody (GTX115646) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min