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Rac1 + CDC42 (phospho Ser71) antibody

Orthogonal
Anti-Rac1 + CDC42 (phospho Ser71) antibody used in Western Blot (WB). GTX25482
Anti-Rac1 + CDC42 (phospho Ser71) antibody used in IHC (Paraffin sections) (IHC-P). GTX25482

Cat. No. GTX25482

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
50 μl ($399)
See all Rac1 + CDC42 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:100
FACS 1:20
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rac1-protein that contains serine 71. The sequence is conserved in human and mouse Rac 1, 2, and 3, and cdc42 human, mouse, rat, dog and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.

Research Area

DATA IMAGES

Anti-Rac1 + CDC42 (phospho Ser71) antibody used in Western Blot (WB). GTX25482

GTX25482 WB Image

Orthogonal

WB (peptide competition) analysis of A431 cells stimulated with EGF (Lane 2-6) using GTX25482 RAC1 + CDC42 (phospho Ser71) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-Rac1 + CDC42 (phospho Ser71) antibody used in IHC (Paraffin sections) (IHC-P). GTX25482

GTX25482 IHC-P Image

IHC-P analysis of human stomach tissue using GTX25482 RAC1 + CDC42 (phospho Ser71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

Application Reference

Lin KT et al. Naunyn Schmiedebergs Arch Pharmacol 2012; 385 (1) : 39-49 Bp5250 inhibits vascular endothelial growth factor-induced angiogenesis and HIF-1α expression on endothelial cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399