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Rac1 antibody

Anti-Rac1 antibody used in Western Blot (WB). GTX100761
Anti-Rac1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100761
Anti-Rac1 antibody used in Western Blot (WB). GTX100761

Cat No. GTX100761

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Sheep, Drosophila, Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rac1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rac family small GTPase 1 , MIG5 , MRD48 , Rac-1 , TC-25 , p21-Rac1

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side , Melanosome

Background

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rac1 antibody used in Western Blot (WB). GTX100761

GTX100761 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX100761 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rac1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100761

GTX100761 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft , using RAC1(GTX100761) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rac1 antibody used in Western Blot (WB). GTX100761

GTX100761 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: Raji
12% SDS PAGE
GTX100761 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169