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Rad1 antibody [4126]

Anti-Rad1 antibody [4126] used in Western Blot (WB). GTX70317

Cat. No. GTX70317

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4126

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Rad1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000

Note :

WB
Detects a band of approximately 28 kDa
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino terminal fragment of human Rad1 purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_386049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD1 checkpoint DNA exonuclease , HRAD1 , REC1

Cellular Localization

Nucleus

Background

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-Rad1 antibody [4126] used in Western Blot (WB). GTX70317

GTX70317 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Rad1 antibody [4126] (GTX70317) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399