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Rad17 (phospho Ser645) antibody [EP1519Y]

Anti-Rad17 (phospho Ser645) antibody [EP1519Y] used in Western Blot (WB). GTX61991

Cat. No. GTX61991

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EP1519Y

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Rad17 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:2500 - 5000
Optimal working dilution for a specific application should be determined by the investigator.

Calculated MW

77 kDa. ( Note )

Product Note

The antibody only detects Rad17 phosphorylated on Serine 645.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

RAD17 checkpoint clamp loader component , CCYC , HRAD17 , R24L , RAD17SP , RAD24

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Rad17 (phospho Ser645) antibody [EP1519Y] used in Western Blot (WB). GTX61991

GTX61991 WB Image

A. Western blot analysis on HeLa cell lysates using anti-Rad17 phospho (pS645) RabMAb (cat. #GTX61991) 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399