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Rad17 (phospho Ser656) antibody

Anti-Rad17 (phospho Ser656) antibody used in IHC (Paraffin sections) (IHC-P). GTX132150
Anti-Rad17 (phospho Ser656) antibody used in Western Blot (WB). GTX132150
Anti-Rad17 (phospho Ser656) antibody used in IHC (Paraffin sections) (IHC-P). GTX132150
Anti-Rad17 (phospho Ser656) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132150

Cat No. GTX132150

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

293T (100 uM etoposide treatment for 2 hrs)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Rad17 (phospho Ser656). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RAD17 checkpoint clamp loader component,CCYC,HRAD17,R24L,RAD17SP,RAD24

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Rad17 (phospho Ser656) antibody used in IHC (Paraffin sections) (IHC-P). GTX132150

GTX132150 IHC-P Image

Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad17 (phospho Ser656) antibody (GTX132150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad17 (phospho Ser656) antibody used in Western Blot (WB). GTX132150

GTX132150 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (GTX132150) diluted at 1:500.

Anti-Rad17 (phospho Ser656) antibody used in IHC (Paraffin sections) (IHC-P). GTX132150

GTX132150 IHC-P Image

Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
Rad17 (phospho Ser656) antibody (GTX132150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad17 (phospho Ser656) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132150

GTX132150 ICC/IF Image

Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad17 (phospho Ser656) protein stained by Rad17 (phospho Ser656) antibody (GTX132150) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169