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Rad21 antibody [HL2171]

Recombinant antibody
Anti-Rad21 antibody [HL2171] used in Western Blot (WB). GTX638175
Anti-Rad21 antibody [HL2171] used in Immunoprecipitation (IP). GTX638175
Anti-Rad21 antibody [HL2171] used in Western Blot (WB). GTX638175
Anti-Rad21 antibody [HL2171] used in IHC (Paraffin sections) (IHC-P). GTX638175
Anti-Rad21 antibody [HL2171] used in IHC (Paraffin sections) (IHC-P). GTX638175

Cat. No. GTX638175

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2171

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rad21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Rad21

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rad21 antibody [HL2171] used in Western Blot (WB). GTX638175

GTX638175 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (GTX638175) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad21 antibody [HL2171] used in Immunoprecipitation (IP). GTX638175

GTX638175 IP Image

Immunoprecipitation of Rad21 protein from HeLa whole cell extract using 5 μg of Rad21 antibody [HL2171] (GTX638175).
Western blot analysis was performed using Rad21 antibody [HL2171] (GTX638175).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-Rad21 antibody [HL2171] used in Western Blot (WB). GTX638175

GTX638175 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL2171] (GTX638175) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad21 antibody [HL2171] used in IHC (Paraffin sections) (IHC-P). GTX638175

GTX638175 IHC-P Image

Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Rad21 stained by Rad21 antibody [HL2171] (GTX638175) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad21 antibody [HL2171] used in IHC (Paraffin sections) (IHC-P). GTX638175

GTX638175 IHC-P Image

Rad21 antibody [HL2171] detects Rad21 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Rad21 stained by Rad21 antibody [HL2171] (GTX638175) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations