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Rad21 antibody

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in Immunoprecipitation (IP). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106012
Anti-Rad21 antibody used in Western Blot (WB). GTX106012
Anti-Rad21 antibody used in Western Blot (WB). GTX106012

Cat. No. GTX106012

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rad21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , K562 , THP-1 , HL-60

Predict Reactivity

Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176384

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RAD21 antibody (GTX106012) diluted at 1:250.
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad21 antibody used in Immunoprecipitation (IP). GTX106012

GTX106012 IP Image

Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody (GTX106012).
Western blot analysis was performed using RAD21 antibody (GTX106012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106012

GTX106012 ICC/IF Image

Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-Rad21 antibody used in Western Blot (WB). GTX106012

GTX106012 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad21 antibody used in Western Blot (WB). GTX106012

GTX106012 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Marek Bartosovic et al. Nat Biotechnol 2021; 39 (7) : 825-835 Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues.
Application : ChIP assay
Reactivity : Mouse
Ochiai H et al. Sci Adv 2020; 6 (25) : eaaz6699 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
Heinz S et al. Cell 2018; 174 (6) : 1522-1536.e22 Transcription Elongation Can Affect Genome 3D Structure.
Gatei M et al. Hum Mol Genet 2014; RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Rad21 antibody Cat. No. GTX106012

Rating
( Average 5 based on 1 users reviews)
Application
ChIP assay(ChIP assay)
( Average 5 based on 1 users reviews)
Anti-Rad21 antibody used in ChIP assay (ChIP assay). GTX106012
Date : Anonymous submitted on 05-Dec-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Sample : HepG2 cells
Amount used : 1 μl chromatin fragments were included in the 200 μl reaction solution (1 mM PIPES-KOH (pH8.0), 8 mM Tris-HCl (pH8.0), 40 mM KCl, 0.2 mM CaCl2, 0.3 mM MgCl2, 0.8 mM EDTA, 112 mM NaCl, 1% sucrose, 0.1% NP40, 0.8% TritonX-100)
Primary Antibody : 1:88, 25°C, 2Hr
Figure Note : ChIP analysis of HepG2 cell lysate using Rad21 antibody (GTX106012). The amount of immunoprecipitated DNA was evaluated by real-time PCR. Y-axis represents the ratios of the immunoprecipitated DNA to the input.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169