GeneTex
United States (US)

Rad21 antibody

GTX106012_43110_20180307_ICC_IF_19011506_184.jpg
GTX106012_40842_IHC_M_19011506_737.jpg
GTX106012_40842_20150317_IP_19011506_244.jpg
GTX106012_43110_20180202_WB_19011506_390.jpg
GTX106012_41864_20151227_IHC-P_19011506_925.jpg
GTX106012_40842_IHC_19011506_811.jpg
GTX106012_40842_WB_M_19011506_480.jpg

Cat No. GTX106012

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rad21 Cohesin Complex Component,Cdls4,Hr21,Hrad21,Mcd1,Nxp1,Scc1,Hhr21,Rad21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106012_43110_20180307_ICC_IF_19011506_184.jpg

GTX106012 ICC/IF Image

Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX106012_40842_IHC_M_19011506_737.jpg

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106012_40842_20150317_IP_19011506_244.jpg

GTX106012 IP Image

Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody (GTX106012).
Western blot analysis was performed using RAD21 antibody (GTX106012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106012_43110_20180202_WB_19011506_390.jpg

GTX106012 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106012_41864_20151227_IHC-P_19011506_925.jpg

GTX106012 IHC-P Image

RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RAD21 antibody (GTX106012) diluted at 1:250.

GTX106012_40842_IHC_19011506_811.jpg

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106012_40842_WB_M_19011506_480.jpg

GTX106012 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX106012 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169