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Rad21 antibody

Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in Immunoprecipitation (IP). GTX106012
Anti-Rad21 antibody used in Western Blot (WB). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012
Anti-Rad21 antibody used in Western Blot (WB). GTX106012

Cat No. GTX106012

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176384

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106012

GTX106012 ICC/IF Image

Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad21 antibody used in Immunoprecipitation (IP). GTX106012

GTX106012 IP Image

Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody (GTX106012).
Western blot analysis was performed using RAD21 antibody (GTX106012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Rad21 antibody used in Western Blot (WB). GTX106012

GTX106012 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RAD21 antibody (GTX106012) diluted at 1:250.

Anti-Rad21 antibody used in IHC (Paraffin sections) (IHC-P). GTX106012

GTX106012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad21 antibody used in Western Blot (WB). GTX106012

GTX106012 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX106012 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169