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Rad21 antibody

Anti-Rad21 antibody used in Western Blot (WB). GTX66677

Cat No. GTX66677

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 521-570 of Human Rad21.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rad21 antibody used in Western Blot (WB). GTX66677

GTX66677 WB Image

WB analysis of various samples using GTX66677 Rad21 antibody.
Lane1 : C6 whole cell lysate(40ug)
Lane2 : 3T3-L1 whole cell lysate(40ug)
Lane3 : Hela whole cell lysate(40ug)
Lane4 : HEK293T whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349