Western blot analysis of Rad22 protein in the whole cell extracts with anti-Rad22 serum before affinity purification. M: Molecular weight markers (kD) Lane1: Wild-type strain Lane2: Rad22 deletion mutant strain

Western blot analysis of Rad22 in the whole cell extracts with affinity-purified antibody. M: Molecular weight markers (kD) Lane 1:Protein size markers (kDa) Lane 2:Extract of wild-type S. pombe indicates Rad22 protein band.