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Rad50 antibody [13B3]

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot and Immunoprecipitation (WB IP). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

Cat No. GTX70228

Host

Mouse

Clonality

Monoclonal

Clone Name

13B3

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, IHC, in vitro

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rad50 Double Strand Break Repair Protein , Nbsld , Rad502 , Hrad50 , Rad50

Cellular Localization

Nuclear

Background

RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions.

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228

GTX70228 ICC/IF Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

The WB analysis of Rad50 antibody [13B3] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

The WB analysis of Rad50 antibody [13B3] was published by Palagyi A and colleagues in the journal Mol Cancer in 2010 . PMID: 20509860

Anti-Rad50 antibody [13B3] used in Western Blot and Immunoprecipitation (WB IP). GTX70228

GTX70228 WB IP Image

The WB, IP analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

The WB analysis of Rad50 antibody [13B3] was published by Mariggiò G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

The WB analysis of Rad50 antibody [13B3] was published by Zhu J and colleagues in the journal EMBO Mol Med in 2013.PMID: 23341130

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329