PRODUCT
Summary
Rad50 antibody detects RAD50, a ~154 kDa protein that, together with MRE11 and NBS1, forms the MRN complex that is intimately involved in DNA damage signaling, DNA double-strand break (DSB) repair, recombination, and replication. The complex is also involved other facets of genomic homeostasis including telomere maintenance. MRN plays a pivotal role in the DNA damage response (DDR) through interactions with ATM and ATR.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HeLa nuclear extract
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Amino acids 1-425 of Rad50 expressed in E. coli.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_372854
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50
Cellular Localization
Nucleus
Background
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
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GTX70228 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 ICC/IF Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
Rad50 antibody [13B3]
Cat. No. GTX70228
Rating
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( Average 5 based on 12 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 11 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2015.
PMID: 26215093
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2015.
PMID: 26215093
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB,IP
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2017.
PMID: 28430817
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal BMC Biol in 2015.
PMID: 25857663
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal EMBO Mol Med in 2013.
PMID: 23341130
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Mol Cancer in 2010.
PMID: 20509860
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Hum Mol Genet in 2015.
PMID: 26085575
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal J Biol Chem in 2013.
PMID: 23525106
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2012.
PMID: 23285190
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2012.
PMID: 23285190
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2017.
PMID: 28430817
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Rep in 2016.
PMID: 27568553