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Rad50 antibody [13B3]

KO/KD Orthogonal
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

Cat. No. GTX70228

Reference 118
12

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 13B3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, IHC, in vitro

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all Rad50 products
PRODUCT

Summary

Rad50 antibody detects RAD50, a ~154 kDa protein that, together with MRE11 and NBS1, forms the MRN complex that is intimately involved in DNA damage signaling, DNA double-strand break (DSB) repair, recombination, and replication. The complex is also involved other facets of genomic homeostasis including telomere maintenance. MRN plays a pivotal role in the DNA damage response (DDR) through interactions with ATM and ATR.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_372854

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228

GTX70228 ICC/IF Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sigismondo G et al. Nucleic Acids Res 2023; 51 (2) : 687-711 Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair.
Dullovi A et al. iScience 2023; 26 (3) : 106107 Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase.
Application : WB
Reactivity : Human
Belmonte-Fern?ndez A et al. Cell Death Differ 2023; 30 (2) : 488-499 Cisplatin-induced cell death increases the degradation of the MRE11-RAD50-NBS1 complex through the autophagy/lysosomal pathway.
Price AM et al. PLoS Pathog 2022; 18 (9) : e1010797 Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts.
Application : WB
Reactivity : Human
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Rad50 antibody [13B3] Cat. No. GTX70228

Rating
( Average 5 based on 12 users reviews)
Application
Western Blot(WB)
( Average 5 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26215093
Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26215093
Anti-Rad50 antibody [13B3] used in Western Blot and Immunoprecipitation (WB IP). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28430817
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015. PMID: 25857663
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010. PMID: 20509860
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Hum Mol Genet in 2015. PMID: 26085575
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biol Chem in 2013. PMID: 23525106
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012. PMID: 23285190
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012. PMID: 23285190
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28430817
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2016. PMID: 27568553
Show More +
Submit a Review
Application Reference
Sigismondo G et al. Nucleic Acids Res 2023; 51 (2):687-711 Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair.
Dullovi A et al. iScience 2023; 26 (3):106107 Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase.
Application : WB
Reactivity : Human
Belmonte-Fern?ndez A et al. Cell Death Differ 2023; 30 (2):488-499 Cisplatin-induced cell death increases the degradation of the MRE11-RAD50-NBS1 complex through the autophagy/lysosomal pathway.
Price AM et al. PLoS Pathog 2022; 18 (9):e1010797 Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts.
Application : WB
Reactivity : Human
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Alexander M Price et al. Nucleic Acids Res 2022; 50 (3):1201-1220 Adenovirus prevents dsRNA formation by promoting efficient splicing of viral RNA.
Application : WB
Reactivity : Human
Boddin J et al. mBio 2022;:e0014422 A Single Amino Acid Switch in the Adenoviral DNA Binding Protein Abrogates Replication Center Formation and Productive Viral Infection.
Eui Tae Kim et al. Nat Microbiol 2021; 6 (2):234-245 Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription.
Application : WB
Reactivity : Human
Josephine Mun Yee Ko et al. Cancers (Basel) 2021; 13 (18) RAD50 Loss of Function Variants in the Zinc Hook Domain Associated with Higher Risk of Familial Esophageal Squamous Cell Carcinoma.
Application : WB
Reactivity : Human
Dybas JM et al. mSystems 2021;:e0046821 Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes.
Application : WB
Reactivity : Human
Sun J et al. Chemosphere 2021; 266:129177 microRNA-21, via the HIF-1α/VEGF signaling pathway, is involved in arsenite-induced hepatic fibrosis through aberrant cross-talk of hepatocytes and hepatic stellate cells.
Application : WB
Reactivity : Human
Herrmann C et al. Nat Microbiol 2020; 5 (10):1217-1231 Adenovirus-mediated ubiquitination alters protein-RNA binding and aids viral RNA processing.
Application : WB
Reactivity : Human
Ly P et al. Nat Genet 2019; 51 (4):705-715 Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements.
Godau J et al. DNA Repair (Amst) 2019; 77:96-108 Identification of a miniature Sae2/Ctp1/CtIP ortholog from Paramecium tetraurelia required for sexual reproduction and DNA double-strand break repair.
Anand R et al. EMBO J 2019; (Epub) NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Application : WB
Reactivity : Human
Fujiwara C et al. Sci Rep 2018; 8 (1):14827 Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells.
Application : WB
Reactivity : Human
Johnson TE et al. Cell Rep 2018; 24 (6):1471-1483 Homeodomain Proteins Directly Regulate ATM Kinase Activity.
Application : WB
Reactivity : Human
Pancholi NJ et al. Virology 2018; 518:221-231 Serotype-specific restriction of wild-type adenoviruses by the cellular Mre11-Rad50-Nbs1 complex.
Application : WB
Reactivity : Human
Lee JH et al. Sci Signal 2018; 11 (512) ATM directs DNA damage responses and proteostasis via genetically separable pathways.
Application : in vitro
Reactivity : Human
Tang JY et al. Environ Toxicol 2017; (Epub) 4β-Hydroxywithanolide E selectively induces oxidative DNA damage for selective killing of oral cancer cells.
Application : WB
Reactivity : Human
Li Z et al. Cancer Res 2017; (Epub) Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.
Application : WB
Reactivity : Human
Mariggio G et al. PLoS Pathog 2017; 13 (4):e1006335 Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency.
Application : WB
Reactivity : Human
Henrich SM et al. Sci Rep 2017; 7:41663 Interplay with the Mre11-Rad50-Nbs1 complex and phosphorylation by GSK3β implicate human B-Myb in DNA-damage signaling.
Application : WB, ICC/IF
Reactivity : Human
Tammaro M et al. Nucleic Acids Res 2016; 44 (1):221-31 DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.
Application : WB
Reactivity : Human
Johnson BA et al. Virology 2016; 500:22-34 The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.
Application : WB
Reactivity : Human
Staples CJ et al. Cell Rep 2016; 16 (10):2565-75 MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.
Application : WB
Reactivity : Human
Kavanaugh G et al. Mol Cell Biol 2015; 35 (17):2979-90 Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.
Application : ICC/IF
Reactivity : Human
Xiong J et al. J Cell Biol 2015; 208 (5):513-20 Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.
Lee KY et al. Nat Commun 2015; 6:7744 MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Virts EL et al. Hum Mol Genet 2015; 24 (18):5093-108 AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia.
Application : WB
Reactivity : Human
Harten SK et al. BMC Biol 2015; 13:21 The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Application : WB
Reactivity : Human
Kinoshita E et al. Biochimie 2015; Human RAD50 makes a functional DNA-binding complex.
Application : WB
Reactivity : Human
Huang YL et al. Hum Mol Genet 2015; 24:3506-17 FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Application : WB
Reactivity : Human
Sanjiv K et al. Transl Oncol 2014; 7 (2):256-266.e5 PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Application : WB
Reactivity : Human
Sanjiv K et al. Translational Oncology 2014; PI3K inhibition augments the therapeutic efficacy of a 3a-aza-cyclopenta[α]indene derivative in lung cancer cells
Application : WB
Reactivity : Human
Ohara M et al. J Radiat Res 2014; Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
Application : WB
Reactivity : Human
Tahara M et al. Mol Cancer Ther 2014; The use of olaparib (AZD2281) potentiates SN-38 cytotoxicity in colon cancer cells by indirect inhibition of Rad51-mediated repair of DNA double-strand breaks.
Application : WB
Reactivity : Human
Kong X et al. Mol Cell Biol 2014; 34 (4):685-98 Distinct Functions of Human Cohesin-SA1 and Cohesin-SA2 in Double-Strand Break Repair.
Application : WB, IP
Reactivity : Human
Bohringer M et al. Oncogene 2013; 32 (48):5458-70 siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors.
Floyd SR et al. Nature 2013; 498 (7453):246-50 The bromodomain protein Brd4 insulates chromatin from DNA damage signalling.
Simoes M et al. Vet Microbiol 2013; 165 (43102):140-7 Host DNA damage response facilitates African swine fever virus infection.
Application : WB
Zhu J et al. EMBO Mol Med 2013; 5 (3):353-65 A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia.
Application : WB
Reactivity : Human
Lee JH et al. J Biol Chem 2013; 288 (18):12840-51 Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex.
Application : WB
Ogiwara H et al. PLoS One 2012; 7 (12):e52810 CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes.
Application : WB
Reactivity : Human
Knies K et al. PLoS One 2012; 7 (12):e52648 Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges.
Application : WB
Reactivity : Human
Oplustilova L et al. Cell Cycle 2012; 11 (20):3837-50 Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Application : WB
Reactivity : Human
Stoepker C et al. Nat Genet 2011; 43 (2):138-41 SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype.
Anderson VE et al. Cancer Res 2011; 71 (2):463-72 CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
Matsumoto Y et al. DNA Repair (Amst) 2011; 10 (3):314-21 Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly.
Application : WB
Reactivity : Human
Luo Y et al. J Virol 2011; 85 (1):133-45 Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
Masuda Y et al. Biochem Biophys Res Commun 2011; 415 (2):305-12 Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin.
Application : WB
Reactivity : Human
Cheng Q et al. Nucleic Acids Res 2011; 39 (22):9605-19 Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.
Application : ICC/IF
Baydoun HH et al. Blood 2011; 117 (22):5897-906 Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair.
Application : ICC/IF
Reactivity : Human
Kuroda S et al. Cancer Res 2010; 70 (22):9339-48 Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery.
Momcilovic O et al. PLoS One 2010; 5 (10):e13410 DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.
Application : WB
Reactivity : Human
Machida K et al. J Immunol 2010; 185 (11):6985-98 Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes.
Application : WB
Balasubramanian N et al. J Virol 2010; 84 (24):12504-14 Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex.
Application : ICC/IF
Gomez-Godinez V et al. Nucleic Acids Res 2010; 38 (22):e202 Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.
Palagyi A et al. Mol Cancer 2010; 9:127 Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents.
Application : WB
Reactivity : Human
Cha H et al. Cancer Res 2010; 70 (10):4112-22 Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response.
Application : ICC/IF
Reactivity : Human
Chailleux C et al. Biochem J 2010; 426 (3):365-71 Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Application : WB
Reactivity : Human
Lee JH et al. EMBO J 2010; 29 (3):574-85 53BP1 promotes ATM activity through direct interactions with the MRN complex.
Application : WB
Moody CA et al. PLoS Pathog 2009; 5 (10):e1000605 Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation.
Application : WB
Reactivity : Human
Waltes R et al. Am J Hum Genet 2009; 84 (5):605-16 Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.
Application : ICC/IF
Reactivity : Human
Schwartz RA et al. J Virol 2009; 83 (12):6269-78 Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase.
Application : WB
Reactivity : Human
Zheng L et al. Nucleic Acids Res 2009; 37 (8):2645-57 MRE11 complex links RECQ5 helicase to sites of DNA damage.
Reactivity : Human
Collaco RF et al. Virology 2009; 392 (1):24-33 Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways.
Application : ICC/IF
Reactivity : Human
Carson CT et al. EMBO J 2009; 28 (6):652-62 Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Reactivity : Human
Bartkova J et al. Mol Oncol 2008; 2 (4):296-316 Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene.
Application : ICC/IF
Reactivity : Human
Schwartz RA et al. J Virol 2008; 82 (18):9043-55 Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates.
Application : WB
Reactivity : Human
Lakdawala SS et al. J Virol 2008; 82 (17):8362-72 Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation.
Reactivity : Human
Cervelli T et al. J Cell Sci 2008; 121 (Pt 3):349-57 Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins.
Gregory DA et al. Virology 2008; 373 (1):124-36 Characterization of mre11 loss following HSV-1 infection.
Application : WB
Reactivity : Human
Liu L et al. Nat Cell Biol 2007; 9 (12):1436-41 Telomere lengthening early in development.
Schwartz RA et al. J Virol 2007; 81 (23):12936-45 The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.
Zhong ZH et al. J Biol Chem 2007; 282 (40):29314-22 Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres.
Application : WB
Bentle MS et al. Cancer Res 2007; 67 (14):6936-45 Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone.
Application : ICC/IF
Reactivity : Human
Potts PR et al. Nat Struct Mol Biol 2007; 14 (7):581-90 The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins.
Cho YW et al. J Biol Chem 2007; 282 (28):20395-406 PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
Bae NS et al. Mol Cell 2007; 26 (3):323-34 A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.
Application : WB
Reactivity : Human
Lin X et al. Mol Cell Biol 2007; 27 (13):4905-16 RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival.
Application : WB
Reactivity : Human
You KT et al. PLoS Biol 2007; 5 (5):e109 Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors.
Application : WB
Fleisig HB et al. Oncogene 2007; 26 (33):4797-805 Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes.
Dote H et al. Cancer Res 2006; 66 (18):9211-20 Inhibition of hsp90 compromises the DNA damage response to radiation.
Application : ICC/IF
Reactivity : Human
Takemura H et al. J Biol Chem 2006; 281 (41):30814-23 Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks.
Application : WB
Reactivity : Human
Kong LJ et al. Mol Cell 2006; 22 (1):63-71 The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1.
Application : WB
Reactivity : Human
Myers JS et al. J Biol Chem 2006; 281 (14):9346-50 Rapid activation of ATR by ionizing radiation requires ATM and Mre11.
Application : IHC
Reactivity : Human
Tommiska J et al. Int J Cancer 2006; 118 (11):369413 Evaluation of RAD50 in familial breast cancer predisposition.
Application : WB
van der Burg M et al. J Clin Invest 2006; 116 (1):137-45 A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation.
Mongiat-Artus P et al. Oncogene 2006; 25 (14):78011 Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract.
Application : IHC
Reactivity : Human
Oishi H et al. J Biol Chem 2006; 281 (1):43271 An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Application : WB
Reactivity : Human
Lilley CE et al. Proc Natl Acad Sci U S A 2005; 102 (16):1440762 DNA repair proteins affect the lifecycle of herpes simplex virus 1.
Reactivity : Human
Fasching CL et al. Cancer Res 2005; 65 (7):300474 Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies.
Application : ICC/IF
Liu Y et al. J Virol 2005; 79 (22):14004-16 Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex.
Application : ICC/IF
Reactivity : Human
Kim JS et al. J Cell Biol 2005; 170 (3):341-7 Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells.
Application : ICC/IF
Reactivity : Human
Koh KH et al. Lab Invest 2005; 85 (9):1130-8 Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas.
Application : WB
Reactivity : Human
van Veelen LR et al. Mutat Res 2005; 574 (43102):22-33 Analysis of ionizing radiation-induced foci of DNA damage repair proteins.
Application : ICC/IF
Angele S et al. J Clin Pathol 2004; 57 (11):1179-84 Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast.
Application : IHC
Reactivity : Human
O'Connor MS et al. J Biol Chem 2004; 279 (27):28585-91 The human Rap1 protein complex and modulation of telomere length.
Application : WB
Reactivity : Human
Wilkinson DE et al. J Virol 2004; 78 (9):4783-96 Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response.
Dork T et al. Am J Med Genet A 2004; 126A (3):272-7 Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations.
Application : WB
Giannini G et al. Oncogene 2004; 23 (15):270463 Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Application : WB
Reactivity : Human
Stewart GS et al. Nature 2003; 421 (6926):961-6 MDC1 is a mediator of the mammalian DNA damage checkpoint.
Anderson LA et al. Mol Cell Biol 2003; 23 (2):721-32 Regulation of RelA (p65) function by the large subunit of replication factor C.
Application : WB
Reactivity : Human
Hassane DC et al. Infect Immun 2003; 71 (1):541-5 Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells.
Application : ICC/IF
Reactivity : Human
Carson CT et al. EMBO J 2003; 22 (24):6610-20 The Mre11 complex is required for ATM activation and the G2/M checkpoint.
Application : WB
Reactivity : Human
Uziel T et al. EMBO J 2003; 22 (20):5612-21 Requirement of the MRN complex for ATM activation by DNA damage.
Application : ICC/IF
Reactivity : Human
Xu X et al. FASEB J 2003; 17 (13):1842-8 NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Application : WB
Reactivity : Human
Angele S et al. Histopathology 2003; 43 (4):347-53 Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas.
Application : IHC
Reactivity : Human
Richie CT et al. Mol Cell Biol 2002; 22 (24):8635-47 hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage.
Application : ICC/IF
Reactivity : Human
Pichierri P et al. Hum Mol Genet 2002; 11 (21):2531-46 DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein.
Application : ICC/IF
Kim JS et al. J Biol Chem 2002; 277 (47):45149-53 Specific recruitment of human cohesin to laser-induced DNA damage.
Application : ICC/IF
Reactivity : Human
Stracker TH et al. Nature 2002; 418 (6895):348-52 Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
Application : ICC/IF
Reactivity : Human
Franchitto A et al. J Cell Biol 2002; 157 (1):19-30 Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
Application : ICC/IF
Tauchi H et al. J Biol Chem 2001; 276 (1):43439 The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity.
Application : ICC/IF
Reactivity : Human
Wang Y et al. Genes Dev 2000; 14 (8):927-39 BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Application : ICC/IF
Reactivity : Human
Harfst E et al. Mol Immunol 2000; 37 (15):915-29 Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Application : WB
Paull TT et al. Curr Biol 2000; 10 (15):886-95 A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.
Application : ICC/IF
Reactivity : Human
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