Rad50 antibody detects RAD50, a ~154 kDa protein that, together with MRE11 and NBS1, forms the MRN complex that is intimately involved in DNA damage signaling, DNA double-strand break (DSB) repair, recombination, and replication. The complex is also involved other facets of genomic homeostasis including telomere maintenance. MRN plays a pivotal role in the DNA damage response (DDR) through interactions with ATM and ATR.
*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , HeLa nuclear extract
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.7 mg/ml (Please refer to the vial label for the specific concentration.)
Amino acids 1-425 of Rad50 expressed in E. coli.
Affinity purified by Protein G.
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]