GeneTex
  • Country / Location Selection

United States (US)

Rad50 antibody [13B3]

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228
Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228
Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

Cat No. GTX70228

Host

Mouse

Clonality

Monoclonal

Clone Name

13B3

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, IHC, in vitro

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)
PRODUCT

Summary

Rad50 antibody detects RAD50, a ~154 kDa protein that, together with MRE11 and NBS1, forms the MRN complex that is intimately involved in DNA damage signaling, DNA double-strand break (DSB) repair, recombination, and replication. The complex is also involved other facets of genomic homeostasis including telomere maintenance. MRN plays a pivotal role in the DNA damage response (DDR) through interactions with ATM and ATR.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_372854

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad50 antibody [13B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70228

GTX70228 ICC/IF Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in IHC (Paraffin sections) (IHC-P). GTX70228

GTX70228 IHC-P Image

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rad50 antibody [13B3] used in Western Blot (WB). GTX70228

GTX70228 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Rad50 antibody [13B3] Cat No. GTX70228

Rating ( Average 5 based on 12 users reviews)
Application
Western Blot(WB) ( Average 5 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26215093
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26215093
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28430817
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015.PMID: 25857663
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013.PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010.PMID: 20509860
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Hum Mol Genet in 2015.PMID: 26085575
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biol Chem in 2013.PMID: 23525106
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012.PMID: 23285190
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2012.PMID: 23285190
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28430817
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2016.PMID: 27568553
Application Reference
SDS
PBS.pdf