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Rad50 antibody [N1N2], N-term

KO/KD Orthogonal
Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731
Anti-Rad50 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX119731

Cat. No. GTX119731

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rad50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

Neuro2A , GL261 , NT2D1 , HeLa nuclear extract , PC-12 , HeLa

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618353

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Cellular Localization

Nucleus , Telomere

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731

GTX119731 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731

GTX119731 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731

GTX119731 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX119731 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731

GTX119731 WB Image

Rad50 antibody [N1N2], N-term detects RAD50 protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg GL261 whole cell lysate/extract
5% SDS-PAGE
Rad50 antibody [N1N2], N-term (GTX119731) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad50 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119731

GTX119731 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rad50(GTX119731) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad50 antibody [N1N2], N-term used in Western Blot (WB). GTX119731

GTX119731 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rad50 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX119731

GTX119731 IP Image

Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [N1N2], N-term (GTX119731).
Western blot analysis was performed using Rad50 antibody [N1N2], N-term (GTX119731).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

REFERENCE

Application Reference

Heiser K et al. Virology 2016; 497 : 346-56 Activation of DNA damage repair pathways by murine polyomavirus.
Application : WB
Reactivity : Mouse
Robert Garcea MD et al. 0 2015; Mouse Polyomavirus T Antigens: Directors of Cell Cycle Signaling
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169