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Rad50 antibody

Anti-Rad50 antibody used in Western Blot (WB). GTX32832
Anti-Rad50 antibody used in Immunoprecipitation (IP). GTX32832

Cat No. GTX32832

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP Assay dependent
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAD50 (NP_005723.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody used in Western Blot (WB). GTX32832

GTX32832 WB Image

WB analysis of various samples using GTX32832 Rad50 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Rad50 antibody used in Immunoprecipitation (IP). GTX32832

GTX32832 IP Image

IP analysis of MCF7 cell lysate using GTX32832 Rad50 antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:500

REFERENCE

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REVIEW

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Package List Price ($)
$ 329