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Rad51 antibody [14B4]

Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

Cat No. GTX70230

Host Mouse
Clonality Monoclonal
Clone Name 14B4
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, PLA
Reactivity Human, Mouse, Rat, Chicken
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length (amino acids 1-338) Rad51 expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rad51 Recombinase,Brcc5,Fancr,Hrad51,Hsrad51,Hst16930,Mrmv2,Rad51A,Reca,Rad51

Background

Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Database

Research Area

DATA IMAGES
Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230

GTX70230 IP Image

Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230

GTX70230 ICC/IF Image

Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (GTX70230).
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.

Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230

GTX70230 IHC-P Image

RAD51 antibody 14B4 (GTX70230) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (GTX702308) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (70 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW

Rad51 antibody [14B4] Cat No. GTX70230

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 23-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 80 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1.5Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319