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Rad51 antibody [14B4]

Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230

Cat No. GTX70230

Host

Mouse

Clonality

Monoclonal

Clone Name

14B4

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IP, IHC, PLA

Reactivity

Human, Mouse, Rat, Chicken
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length (amino acids 1-338) Rad51 expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_372856

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion matrix

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230

GTX70230 IP Image

Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230

GTX70230 ICC/IF Image

Rad51 antibody [14B4] detects Rad51 protein at nuclear foci by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 stained by Rad51 antibody [14B4] (GTX70230) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230

GTX70230 ICC/IF Image

Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (GTX70230).
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.

Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230

GTX70230 IHC-P Image

RAD51 antibody 14B4 (GTX70230) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (GTX702308) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Rad51 antibody [14B4] Cat No. GTX70230

Rating ( Average 4.9 based on 17 users reviews)
Application
Western Blot(WB) ( Average 4.9 based on 10 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2017 in Cell. PMID: 28575672
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Int J Radiat Oncol Biol Phys in 2016.PMID: 27020103
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2019.PMID: 31080552
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26727879
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2018.PMID: 29985131
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26727879
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25310191
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 30177840
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 30177840
Date : Anonymous submitted on 23-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 80 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1.5Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 379