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Rad51 antibody [14B4]

Recombinant antibody KO/KD Orthogonal
Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

Cat. No. GTX70230

Reference 97
17

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14B4

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, IHC, PLA

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μl ($399)
See all Rad51 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length (amino acids 1-338) Rad51 expressed in E. coli.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_372856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion matrix

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody [14B4] used in Immunoprecipitation (IP). GTX70230

GTX70230 IP Image

Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230

GTX70230 ICC/IF Image

Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (GTX70230).
Cells were pre-extracted with CSK buffer before fixation with 4% PFA.
RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus.
Scale bar, 10 mciro-meter.

Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230

GTX70230 ICC/IF Image

Orthogonal

Rad51 antibody [14B4] detects Rad51 protein at nuclear foci by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 stained by Rad51 antibody [14B4] (GTX70230) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230

GTX70230 IHC-P Image

RAD51 antibody 14B4 (GTX70230) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (GTX702308) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230

GTX70230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yuan M et al. J Nanobiotechnology 2023; 21 (1) : 393 A carrier-free supramolecular nano-twin-drug for overcoming irinotecan-resistance and enhancing efficacy against colorectal cancer.
van Wijk LM et al. Breast Cancer Res Treat 2023; 202 (3) : 607-616 Performance of a RAD51-based functional HRD test on paraffin-embedded breast cancer tissue.
Lin IC et al. Cells 2023; 12 (19) Role of Nucleobindin-2 in the Clinical Pathogenesis and Treatment Resistance of Glioblastoma.
J Vollmer et al. J Neurooncol 2023; Class I HDAC inhibition reduces DNA damage repair capacity of MYC-amplified medulloblastoma cells
Application : WB
Park J et al. NAR Cancer 2023; 5 (3) : zcad050 INHAT subunit SET/TAF-Iβ regulates PRC1-independent H2AK119 mono-ubiquitination via E3 ligase MIB1 in colon cancer.
Show More +
Submit a Reference

REVIEW

Rad51 antibody [14B4] Cat. No. GTX70230

Rating
( Average 4.9 based on 17 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 10 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2017 in Cell. PMID: 28575672
Anti-Rad51 antibody [14B4] used in IHC (Paraffin sections) (IHC-P). GTX70230
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Int J Radiat Oncol Biol Phys in 2016.PMID: 27020103
Anti-Rad51 antibody [14B4] used in Immunohistochemistry (IHC). GTX70230
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2019.PMID: 31080552
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26727879
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2018.PMID: 29985131
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26727879
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25310191
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23341130
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Oncol in 2017. PMID: 28173629
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 30177840
Anti-Rad51 antibody [14B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70230
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 30177840
Anti-Rad51 antibody [14B4] used in Western Blot (WB). GTX70230
Date : Anonymous submitted on 23-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 80 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1.5Hr
Show More +
Submit a Review
Application Reference
Yuan M et al. J Nanobiotechnology 2023; 21 (1):393 A carrier-free supramolecular nano-twin-drug for overcoming irinotecan-resistance and enhancing efficacy against colorectal cancer.
van Wijk LM et al. Breast Cancer Res Treat 2023; 202 (3):607-616 Performance of a RAD51-based functional HRD test on paraffin-embedded breast cancer tissue.
Lin IC et al. Cells 2023; 12 (19) Role of Nucleobindin-2 in the Clinical Pathogenesis and Treatment Resistance of Glioblastoma.
J Vollmer et al. J Neurooncol 2023; Class I HDAC inhibition reduces DNA damage repair capacity of MYC-amplified medulloblastoma cells
Application : WB
Park J et al. NAR Cancer 2023; 5 (3):zcad050 INHAT subunit SET/TAF-Iβ regulates PRC1-independent H2AK119 mono-ubiquitination via E3 ligase MIB1 in colon cancer.
Miaomiao Yuan et al. Research Square 2023; A Carrier-Free Supramolecular Nano-Twin-Drug for Overcoming Irinotecan- Resistance and Enhancing Efficacy against Colorectal Cancer
Application : ICC/IF
Ter Brugge P et al. Nat Commun 2023; 14 (1):1958 Homologous recombination deficiency derived from whole-genome sequencing predicts platinum response in triple-negative breast cancers.
Application : IHC-P
Reactivity : Mouse
Capala ME et al. Cancers (Basel) 2023; 15 (2) Ex Vivo Functional Assay for Evaluating Treatment Response in Tumor Tissue of Head and Neck Squamous Cell Carcinoma.
Lu X et al. J Exp Clin Cancer Res 2022; 41 (1):355 CBL0137 impairs homologous recombination repair and sensitizes high-grade serous ovarian carcinoma to PARP inhibitors.
Cheng YW et al. J Oncol 2022; 2022:7250278 miR-3059-3p Regulates Glioblastoma Multiforme Radiosensitivity Enhancement through the Homologous Recombination Pathway of DNA Repair.
Tanaka K et al. Sci Rep 2022; 12 (1):14764 Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation.
Heijink AM et al. Nat Commun 2022; 13 (1):6722 Sister chromatid exchanges induced by perturbed replication can form independently of BRCA1, BRCA2 and RAD51.
Application : WB
Reactivity : Human
Tsaridou S et al. EMBO Rep 2022; 23 (8):e54483 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.
Application : ICC/IF
Reactivity : Human
Zimmerli D et al. Nat Commun 2022; 13 (1):6579 MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling.
Durinikova E et al. Clin Cancer Res 2022; 28 (17):3874-3889 Targeting the DNA Damage Response Pathways and Replication Stress in Colorectal Cancer.
Anna R Michmerhuizen et al. NPJ Breast Cancer 2022; 8 (1):31 Estrogen receptor inhibition mediates radiosensitization of ER-positive breast cancer models.
Application : ICC/IF
Reactivity : Human
Cosgrove N et al. Nat Commun 2022; 13 (1):514 Mapping molecular subtype specific alterations in breast cancer brain metastases identifies clinically relevant vulnerabilities.
Application : IHC-P
Reactivity : Human
Jiehui Deng et al. Nat Cancer 2021; 2 (5):503-514 ULK1 inhibition overcomes compromised antigen presentation and restores antitumor immunity in LKB1 mutant lung cancer.
Application : WB
Reactivity : Human
Lise M van Wijk et al. Cancers (Basel) 2021; 13 (12) The RAD51-FFPE Test; Calibration of a Functional Homologous Recombination Deficiency Test on Diagnostic Endometrial and Ovarian Tumor Blocks.
Application : IHC-P
Reactivity : Human
Pesch AM et al. JCI Insight 2021; (Epub) RB expression confers sensitivity to CDK4/6 inhibitor-mediated radiosensitization across breast cancer subtypes.
Application : ICC/IF
Reactivity : Human
Lee M et al. Nucleic Acids Res 2021; 49 (10):5588-5604 Cancer-causing BRCA2 missense mutations disrupt an intracellular protein assembly mechanism to disable genome maintenance.
Application : WB
Reactivity : Human
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : ICC/IF
Reactivity : Human
Bold I.T. et al. Cancers 2021; 13 (6):1194 DNA Damage Response during Replication Correlates with CIN70 Score and Determines Survival in HNSCC Patients.
Application : WB
Zhu Y et al. Prostate 2021; 81 (1):81-88 CHD1 and SPOP synergistically protect prostate epithelial cells from DNA damage.
Application : ICC/IF
Reactivity : Human
Coussy F et al. Sci Transl Med 2020; 12 (531) BRCAness, SLFN11, and RB1 loss predict response to topoisomerase I inhibitors in triple-negative breast cancers.
Liang ML et al. Cancers (Basel) 2020; 12 (12) Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas.
Application : WB
Reactivity : Human
Du Y et al. ACS Appl Mater Interfaces 2020; 12 (51):56874-56885 Radiosensitization Effect of AGuIX, a Gadolinium-Based Nanoparticle, in Nonsmall Cell Lung Cancer.
Application : WB
Reactivity : Human
van Wijk LM et al. Cancers (Basel) 2020; 12 (10) The RECAP Test Rapidly and Reliably Identifies Homologous Recombination-Deficient Ovarian Carcinomas.
Chopra N et al. Nat Commun 2020; 11 (1):2662 Homologous recombination DNA repair deficiency and PARP inhibition activity in primary triple negative breast cancer.
Application : IHC-P
Reactivity : Human
Hu Z et al. EMBO J 2020; 39 (12):e104133 BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.
Application : WB
Reactivity : Human
Meyer F et al. Cells 2020; 9 (1) Prevention of DNA Replication Stress by CHK1 Leads to Chemoresistance Despite a DNA Repair Defect in Homologous Recombination in Breast Cancer.
Application : WB
Neuh?user K et al. Clin Transl Radiat Oncol 2019; 15:99-107 Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling.
Perl AL et al. J Biol Chem 2019; 294 (45):17043-17059 Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45).
Application : ICC/IF
Reactivity : Mouse
Krajewska M et al. Nat Commun 2019; 10 (1):1757 CDK12 loss in cancer cells affects DNA damage response genes through premature cleavage and polyadenylation.
Heijink AM et al. Cell Rep 2019; 28 (9):2345-2357.e5
Application : ICC/IF
Reactivity : Human
Kopper O et al. Nat Med 2019; 25 (5):838-849 An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity.
Application : ICC/IF
Reactivity : Human
Ordonez LD et al. Oncotarget 2019; 10 (27):2586-2606 Rapid activation of epithelial-mesenchymal transition drives PARP inhibitor resistance in Brca2-mutant mammary tumours.
Application : ICC/IF
Heijink AM et al. Nat Commun 2019; 10 (1):100 BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity.
Application : ICC/IF
Reactivity : Human
Marzio A et al. Mol Cell 2019; 73 (2):224-237.e6 The F-Box Domain-Dependent Activity of EMI1 Regulates PARPi Sensitivity in Triple-Negative Breast Cancers.
Application : WB
Reactivity : Human
Mansour WY et al. Sci Rep 2018; 8 (1):3947 Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer.
Rona G et al. Elife 2018; 7 PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.
Johnson TI et al. Cell Death Dis 2018; 9 (9):913 Fumarate hydratase loss promotes mitotic entry in the presence of DNA damage after ionising radiation.
van den Tempel N et al. PLoS One 2018; 13 (12):e0209101 Ex vivo assays to predict enhanced chemosensitization by hyperthermia in urothelial cancer of the bladder.
Application : IHC-P
Reactivity : Human
Chen CC et al. Cancers (Basel) 2018; 10 (9) Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
Application : WB, ICC/IF
Reactivity : Human
van Gijn SE et al. Oncogene 2018; (Epub) TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells.
Application : ICC/IF
Reactivity : Human
Chen CC et al. Cell Death Dis 2018; 9 (5):543 Corylin increases the sensitivity of hepatocellular carcinoma cells to chemotherapy through long noncoding RNA RAD51-AS1-mediated inhibition of DNA repair.
Application : WB
Reactivity : Human
Baranes-Bachar K et al. Mol Cell 2018; 69 (5):866-878.e7 The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.
Application : ICC/IF
Reactivity : Human
Schoonen PM et al. Nat Commun 2017; 8:15981 Progression through mitosis promotes PARP inhibitor-induced cytotoxicity in homologous recombination-deficient cancer cells.
Application : WB, ICC/IF
Reactivity : Human
Kalimutho M et al. Mol Oncol 2017; 11 (5):470-490 Enhanced dependency of KRAS-mutant colorectal cancer cells on RAD51-dependent homologous recombination repair identified from genetic interactions in Saccharomyces cerevisiae.
Briest F et al. Neuroendocrinology 2017; (Epub) Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors.
Application : WB
Reactivity : Human
Tan SLW et al. Cell 2017; 169 (6):1105-1118.e15 A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability.
Application : WB
Reactivity : Human
Browning CL et al. Toxicol Appl Pharmacol 2017; (Epub) Prolonged exposure to particulate chromate inhibits RAD51 nuclear import mediator proteins.
Application : PLA
Weeden CE et al. PLoS Biol 2017; 15 (1):e2000731 Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway.
Application : IHC-P
Reactivity : Human, Mouse
Reindl J et al. Sci Rep 2017; 7:40616 Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci.
Application : ICC/IF
Reactivity : Human
Tan W et al. Cancer Med 2017; (Epub) Overexpression of TNKS1BP1 in lung cancers and its involvement in homologous recombination pathway of DNA double-strand breaks.
Application : ICC/IF
Reactivity : Human
Jiang Y et al. Int J Radiat Oncol Biol Phys 2016; 95 (2):772-81 Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality.
Application : IHC-P
Reactivity : Human
Zhang H et al. Nat Commun 2016; 7:10201 A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice.
Application : ICC/IF
Reactivity : Human
Magnus T. Dillon et al. Mol. Cancer Ther. 2016; (Epub) Radiosensitization by the ATR inhibitor AZD6738 through generation of acentric micronuclei.
Application : IHC-P
Reactivity : Human
Bhide SA et al. Br J Cancer 2016; 115 (7):825-30 Delayed DNA double-strand break repair following platin-based chemotherapy predicts treatment response in head and neck squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Schaaf L et al. Cancer Res 2016; (Epub) Hyperthermia synergizes with chemotherapy by inhibiting PARP1-dependent DNA replication arrest.
Application : ICC/IF
Reactivity : Human
Boucher D et al. FASEB J 2015; 29 (8):3326-34 Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.
Application : ICC/IF
Reactivity : Mouse
Bowen C et al. Cancer Res 2015; 75 (13):2686-98 NKX3.1 Suppresses TMPRSS2-ERG Gene Rearrangement and Mediates Repair of Androgen Receptor-Induced DNA Damage.
Application : WB
Reactivity : Human
Luo K et al. Nucleic Acids Res 2015; 43 (11):5465-75 CDK-mediated RNF4 phosphorylation regulates homologous recombination in S-phase.
Application : ICC/IF
Reactivity : Human
Magin S et al. Mol Cancer Ther 2015; 14 (6):1424-33 Inhibition of Homologous Recombination and Promotion of Mutagenic Repair of DNA Double-Strand Breaks Underpins Arabinoside-Nucleoside Analogue Radiosensitization.
Application : ICC/IF
Reactivity : Human
Kausar T et al. Neoplasia 2015; 17 (10):757-66 sensitization of pancreatic cancers to * chemoradiation by wee1 kinase inhibition depends on homologous recombination repair.
Application : ICC/IF
Reactivity : Human
Zhu J et al. Eur J Med Chem 2015; 96:196-208 Synthesis, molecular modeling, and biological evaluation of novel RAD51 inhibitors.
Reactivity : Human
Waddell N et al. Nature 2015; 518 (7540):495-501 Whole genomes redefine the mutational landscape of pancreatic cancer.
Reactivity : Human
Beeharry N et al. Cell Cycle 2014; 13 (14):2172-91 Re-purposing clinical kinase inhibitors to enhance chemosensitivity by overriding checkpoints.
Application : ICC/IF
Reactivity : Human
Sanjiv K et al. Transl Oncol 2014; 7 (2):256-266.e5 PI3K Inhibition Augments the Therapeutic Efficacy of a 3a-aza-Cyclopenta[α]indene Derivative in Lung Cancer Cells.
Application : WB
Reactivity : Human
Bhattacharjee V et al. Cell Cycle 2014; 13 (24):3839-56 a synthetic lethal screen identifies the vitamin d receptor as a novel * sensitizer in pancreatic cancer cells.
Uringa EJ et al. DNA Repair (Amst) 2014; 25C:27-40 A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity.
Application : ICC/IF
Reactivity : Mouse
White MK et al. PLoS One 2014; 9 (10):e110122 Rad51 Activates Polyomavirus JC Early Transcription.
Application : WB
Reactivity : Human
Pefani DE et al. Nat Cell Biol 2014; RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2.
Application : WB
Reactivity : Mouse
Karnak D et al. Clin Cancer Res 2014; Combined Inhibition of Wee1 and PARP1/2 for Radiosensitization in Pancreatic Cancer.
Application : ICC/IF
Reactivity : Human
Naipal KA et al. Clin Cancer Res 2014; Functional ex vivo assay to select Homologous Recombination deficient breast tumors for PARP inhibitor treatment.
Application : IHC-P
Reactivity : Human
Kaminski R et al. PLoS One 2014; 9 (5):e98304 Interplay of Rad51 with NF-κB Pathway Stimulates Expression of HIV-1.
Application : ICC/IF
Reactivity : Human
Sanjiv K et al. Translational Oncology 2014; PI3K inhibition augments the therapeutic efficacy of a 3a-aza-cyclopenta[α]indene derivative in lung cancer cells
Application : WB
Reactivity : Human
Jeyasekharan AD et al. Nat Struct Mol Biol 2013; 20 (10):1191-8 A cancer-associated BRCA2 mutation reveals masked nuclear export signals controlling localization.
Application : WB
Reactivity : Human
Helchowski CM et al. Cell Cycle 2013; 12 (24):3749-58 A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci.
Application : ICC/IF
Reactivity : Human
Pfaffle HN et al. Cancer Res 2013; 73 (20):6254-63 EGFR-activating mutations correlate with a Fanconi anemia-like cellular phenotype that includes PARP inhibitor sensitivity.
Application : ICC/IF
Reactivity : Human
Engelke CG et al. Clin Cancer Res 2013; 19 (16):4412-21 Sensitization of pancreatic cancer to chemoradiation by the Chk1 inhibitor MK8776.
Application : ICC/IF
Reactivity : Human
Wei D et al. Clin Cancer Res 2013; 19 (16):4422-32 Inhibition of protein phosphatase 2A radiosensitizes pancreatic cancers by modulating CDC25C/CDK1 and homologous recombination repair.
Application : ICC/IF
Reactivity : Human
Zhu J et al. EMBO Mol Med 2013; 5 (3):353-65 A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia.
Application : WB
Reactivity : Human
Borst GR et al. Int J Radiat Oncol Biol Phys 2013; 85 (4):1110-8 Targeted radiosensitization by the Chk1 inhibitor SAR-020106.
Application : ICC/IF
Reactivity : Human
Sharma S et al. Nucleic Acids Res 2012; 40 (2):682-91 REV1 and polymerase ζ facilitate homologous recombination repair.
Application : ICC/IF
Reactivity : Human
Oplustilova L et al. Cell Cycle 2012; 11 (20):3837-50 Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment.
Application : ICC/IF
Reactivity : Human
Lee M et al. Oncogene 2011; 30 (30):533500 A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor.
Application : WB
Reactivity : Human
Klammer H et al. Cancer Res 2010; 70 (21):8498-506 Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells.
Application : ICC/IF
Bowen C et al. Cancer Res 2010; 70 (8):3089-97 NKX3.1 activates cellular response to DNA damage.
Application : ICC/IF
Reactivity : Human
Suzuki K et al. Nucleic Acids Res 2010; 38 (12):e129 A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
Application : ICC/IF
Reactivity : Human
Ammazzalorso F et al. EMBO J 2010; 29 (18):3156-69 ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery.
Application : ICC/IF
Graeser M et al. Clin Cancer Res 2010; 16 (24):6159-68 A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer.
Application : ICC/IF
Reactivity : Human
Dungey FA et al. Mol Cancer Ther 2009; 8 (8):2243-54 Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90.
Application : WB
Reactivity : Human
Carlessi L et al. Cell Death Differ 2009; 16 (6):795-806 DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Application : WB
Reactivity : Human
Warren M et al. Hum Mol Genet 2003; 12 (20):2645-56 Phenotypic effects of heterozygosity for a BRCA2 mutation.
Application : ICC/IF
Reactivity : Chicken
Kao GD et al. J Cell Biol 2003; 160 (7):1017-27 Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response.
Application : ICC/IF
Reactivity : Human
Masson JY et al. EMBO J 1999; 18 (22):6552-60 The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Application : WB
Reactivity : Human
SDS
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