GeneTex
United States (US)

Rad51 antibody [N1C2]

KO/KD
GTX100469_40086_20160908_WB_shRNA_watermark_19011506_249.jpg
GTX100469_40086_20160120_IFA_19011506_822.jpg
GTX100469_42711_20170209_IHC-P_H_19011506_964.jpg
GTX100469_40086_20170330_WB_R_19011506_883.jpg
GTX100469_40086_IP_19011506_908.jpg
GTX100469_42711_20170105_WB_19011506_582.jpg
GTX100469_40086_20170330_WB_M_19011506_476.jpg
GTX100469_42711_20181026_WB_Z_19011519_682.jpg

Cat No. GTX100469

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, RAD51A antibody, RECA antibody, RAD51 antibody, "BRCA1/BRCA2-containing complex, subunit 5 antibody", DNA repair protein RAD51 homolog 1 antibody, "RecA, E. coli, homolog of antibody", "RAD51 homolog (RecA homolog, E. coli) antibody", recombination protein A antibody, RAD51 homolog A antibody, RecA-like protein antibody, "RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody"

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100469_40086_20160908_WB_shRNA_watermark_19011506_249.jpg

GTX100469 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469_40086_20160120_IFA_19011506_822.jpg

GTX100469 ICC/IF Image

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 protein stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX100469_42711_20170209_IHC-P_H_19011506_964.jpg

GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100469_40086_20170330_WB_R_19011506_883.jpg

GTX100469 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469_40086_IP_19011506_908.jpg

GTX100469 IP Image

Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rad51 protein by 4 μg Rad51 antibody [N1C2] (GTX100469)
5 % SDS-PAGE
The immunoprecipitated Rad51 protein was detected by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100469_42711_20170105_WB_19011506_582.jpg

GTX100469 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.

GTX100469_40086_20170330_WB_M_19011506_476.jpg

GTX100469 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469_42711_20181026_WB_Z_19011519_682.jpg

GTX100469 WB Image

Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW

Rad51 antibody [N1C2] Cat No. GTX100469

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : H1299 cells
Blocking : 1% BSA in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Trition X-100 in PBS
Primary Antibody : 1:400, 37°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169