Rad51 antibody [N1C2]

Name: Rad51 antibody [N1C2]
Brand: GeneTex
SKU: GTX100469
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469

Anti-Rad51 antibody [N1C2] used in Immunoprecipitation (IP). GTX100469

Anti-Rad51 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100469

Anti-Rad51 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100469

Cat. No. GTX100469

Reference 46

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Rad51 products

View product citations for antibody GTX100469 on CiteAb

PRODUCT

Summary

RAD51 antibody detects RAD51 protein, also known as RAD51 homolog A. RAD51 (37 kDa) is the indispensable strand exchange factor for homologous recombination in DNA repair. It is also essential for replication fork processing and for genome stability. This RAD51 antibody is a well-cited rabbit polyclonal validated for many applications.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

PC-12 , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2 , zebrafish brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951602

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]

Database

Gene ID: 5888 RAD51
UniProt: Q06609 RAD51

Research Area

DATA IMAGES

GTX100469 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX100469 IP Image

Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rad51 protein by 4 μg Rad51 antibody [N1C2] (GTX100469)
5 % SDS-PAGE
The immunoprecipitated Rad51 protein was detected by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.

GTX100469 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100469 ICC/IF Image

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 protein stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX100469 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX100469 WB Image

GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.