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Rad51 antibody

Anti-Rad51 antibody used in Western Blot (WB). GTX00719
Anti-Rad51 antibody used in Western Blot (WB). GTX00719
Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00719
Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00719

Cat No. GTX00719

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IP

Reactivity

Human, Chicken, Xenopus, Rodent
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full-size recombinant human Rad51 protein (His-tag removed)

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion matrix

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody used in Western Blot (WB). GTX00719

GTX00719 WB Image

WB analysis of crude extract of Xenopus using GTX00719 Rad51 antibody.
Dilution : 1:1000

Anti-Rad51 antibody used in Western Blot (WB). GTX00719

GTX00719 WB Image

WB analysis of HeLa whole cell lysate using GTX00719 Rad51 antibody.
Dilution : 1:2000

Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00719

GTX00719 ICC/IF Image

ICC/IF analysis of U2OS cells irradiated by X-ray (2 Gy) for 2hrs using GTX00719 Rad51 antibody.
Green : Primary antibody
Blue : Hoechst 33342
Dilution : 1:6000

Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00719

GTX00719 ICC/IF Image

ICC/IF analysis of normal human diploid cells which were irradiated by X-ray (0.5 Gy) for 6 hrs using GTX00719 Rad51 antibody.
Red : Primary antibody
Dilution : 1:100
Fixation : Cold Methanol for 10 min on ice
Permeabilization : 0.5% Triton X-100 for 5 min on ice

REFERENCE

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REVIEW

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Package List Price ($)
$ 319