ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hrs using GTX00720 Rad51 antibody.
Left : 1:10000 for 30 min at 37 degree C
Right : 1:1000 for 30 min at 37 degree C
Green : Primary antibody
Blue : Hoechst 33342
Fixation : 4% PFA for 10 min
Permeabilization : 0.5% Triton X-100 for 5 min

IHC-P analysis of mouse testis tissue section using GTX00720 Rad51 antibody.
Dilution : 1:100

IP analysis of HeLa whole cell lysate using GTX00720 Rad51 antibody.
The amount of HeLa whole cell lysate and protein A-beads both were using 20 μg.
IP antibody : 20 μg
IB antibody : 20 μg

WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : PC12 whloe cell lysate
Dilution : 1:1000
Loading : 80μg
SDS-PAGE : 12.5%

WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : CHO whole cell lysate
Lane 4 : Xenopus laevis (frog) egg.
Dilution : 1:1000
Loading : 40μg