APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:1000-1:5000 |
| 1:2000-1:5000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full-size recombinant human Rad51 protein
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion matrix
Background
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Database
Research Area
DATA IMAGES
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GTX00720 WB Image
WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : CHO whole cell lysate
Lane 4 : Xenopus laevis (frog) egg.
Dilution : 1:1000
Loading : 40μg
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GTX00720 WB Image
WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : PC12 whloe cell lysate
Dilution : 1:1000
Loading : 80μg
SDS-PAGE : 12.5%
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GTX00720 IP Image
IP analysis of HeLa whole cell lysate using GTX00720 Rad51 antibody.
The amount of HeLa whole cell lysate and protein A-beads both were using 20 μg.
IP antibody : 20 μg
IB antibody : 20 μg
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GTX00720 IHC-P Image
IHC-P analysis of mouse testis tissue section using GTX00720 Rad51 antibody.
Dilution : 1:100
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GTX00720 ICC/IF Image
ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hrs using GTX00720 Rad51 antibody.
Left : 1:10000 for 30 min at 37 degree C
Right : 1:1000 for 30 min at 37 degree C
Green : Primary antibody
Blue : Hoechst 33342
Fixation : 4% PFA for 10 min
Permeabilization : 0.5% Triton X-100 for 5 min
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REFERENCE
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REVIEW
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