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Rad51 antibody

Anti-Rad51 antibody used in Immunoprecipitation (IP). GTX00720
Anti-Rad51 antibody used in Western Blot (WB). GTX00720
Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX00720
Anti-Rad51 antibody used in Western Blot (WB). GTX00720
Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00720

Cat No. GTX00720

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IHC-P, IP, Dot, ELISA, ChIP assay

Reactivity

Human, Mouse, Rat, Chicken, Xenopus, Rodent, Chinese Hamster
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Dot 1:1000-1:5000
ELISA 1:2000-1:5000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-size recombinant human Rad51 protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion matrix

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody used in Immunoprecipitation (IP). GTX00720

GTX00720 IP Image

IP analysis of HeLa whole cell lysate using GTX00720 Rad51 antibody.
The amount of HeLa whole cell lysate and protein A-beads both were using 20 μg.
IP antibody : 20 μg
IB antibody : 20 μg

Anti-Rad51 antibody used in Western Blot (WB). GTX00720

GTX00720 WB Image

WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : CHO whole cell lysate
Lane 4 : Xenopus laevis (frog) egg.
Dilution : 1:1000
Loading : 40μg

Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX00720

GTX00720 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00720 Rad51 antibody.
Dilution : 1:100

Anti-Rad51 antibody used in Western Blot (WB). GTX00720

GTX00720 WB Image

WB analysis of various samples using GTX00720 Rad51 antibody.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : PC12 whloe cell lysate
Dilution : 1:1000
Loading : 80μg
SDS-PAGE : 12.5%

Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00720

GTX00720 ICC/IF Image

ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hrs using GTX00720 Rad51 antibody.
Left : 1:10000 for 30 min at 37 degree C
Right : 1:1000 for 30 min at 37 degree C
Green : Primary antibody
Blue : Hoechst 33342
Fixation : 4% PFA for 10 min
Permeabilization : 0.5% Triton X-100 for 5 min

REFERENCE

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Package List Price ($)
$ 359