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Rad51 antibody

Anti-Rad51 antibody used in Western Blot (WB). GTX00721
Anti-Rad51 antibody used in (). GTX00721
Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00721

Cat. No. GTX00721

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IP

Reactivity

 Poecilia formosa, Poecilia mexicana, Poecilia latipinna, Human, Mouse, Rat, Hamster
Package
100 μg ($399)
See all Rad51 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Phosphate buffer (pH7.4), 100mM NaCl, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-size human red51 protein, no tag peptide attached.

Purification

Purified from the eggs laid by immunized chicken

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion matrix

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody used in Western Blot (WB). GTX00721

GTX00721 WB Image

WB analysis of HeLa whole cell lysate using GTX00721 Rad51 antibody.
Dilution : 1:1000

Anti-Rad51 antibody used in (). GTX00721

GTX00721 Image

SDS-PAGE analysis of the purified IgY.

Anti-Rad51 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00721

GTX00721 ICC/IF Image

ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hr using GTX00721 Rad51 antibody.
Left : 1:100 dilution for 30 min at 37 degree C
Right : 1:1000 for 30 min at 37 degree C
Fixation : 4% PFA for 10 min
Permeabilization : 0.5% Triton X-100 for 5 min

REFERENCE

Application Reference

Dedukh D et al. Chromosome Res 2022; 30 (4) : 443-457 Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa.
Application : ICC/IF
Reactivity : Poecilia formosa, Poecilia mexicana, Poecilia latipinna
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REVIEW

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Application Reference
Dedukh D et al. Chromosome Res 2022; 30 (4):443-457 Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa.
Application : ICC/IF
Reactivity : Poecilia formosa, Poecilia mexicana, Poecilia latipinna
SDS
Glycerol.pdf
Package List Price ($)
$ 399