APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Assay dependent |
Assay dependent |
1 μg / 10⁶ cells |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAD51 paralog C , BROVCA3 , FANCO , R51H3 , RAD51L2
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion
Background
This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX30137 WB Image
WB analysis of (1) HeLa, (2) HepG2, (3) COS-7, and (4) HEK293 cell lysate using GTX30137 Rad51C antibody [2H11/6].
|
|
GTX30137 FACS Image
FACS (Intracellular staining) analysis of HeLa cells using GTX30137 Rad51C antibody [2H11/6]. Blue : Primary antibody Orange : isotype control Dilution : 1 μg/10⁶ cells
|
|
GTX30137 WB Image
WB analysis of HepG2 cell lysate using GTX30137 Rad51C antibody [2H11/6].
|
REFERENCE
There are currently no references for Rad51C antibody [2H11/6] (GTX30137). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rad51C antibody [2H11/6] (GTX30137). Be the first to share your experience with this product.