GeneTex
United States (US)

Rad51C antibody [2H11/6]

GTX30137_95_FACS_18121410_944.jpg
GTX30137_1342_WB_18121410_939.jpg
GTX30137_1341_WB_18121410_945.jpg

Cat No. GTX30137

Host Mouse
Clonality Monoclonal
Clone Name 2H11/6
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, MS
Reactivity Human, Mouse, Yeast, Monkey, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS 1 μg / 10⁶ cells
IP Assay dependent
MS Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rad51 Paralog C,Brovca3,Fanco,R51H3,Rad51L2,Rad51C

Cellular Localization

Nucleus

Background

This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX30137_95_FACS_18121410_944.jpg

GTX30137 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX30137 Rad51C antibody [2H11/6].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/10⁶ cells

GTX30137_1342_WB_18121410_939.jpg

GTX30137 WB Image

WB analysis of HepG2 cell lysate using GTX30137 Rad51C antibody [2H11/6].

GTX30137_1341_WB_18121410_945.jpg

GTX30137 WB Image

WB analysis of (1) HeLa, (2) HepG2, (3) COS-7, and (4) HEK293 cell lysate using GTX30137 Rad51C antibody [2H11/6].

Package List Price ($)
$ 339