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Rad51C antibody, N-term

Anti-Rad51C antibody, N-term used in Western Blot (WB). GTX89889

Cat. No. GTX89889

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Rad51C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89889-PEP).

Product Note

This antibody is expected to recognise all reported human isoforms.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RGKTFRFEMQRDL-C, from the N Terminus of the protein sequence according to NP_002867.1; NP_478123.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 paralog C , BROVCA3 , FANCO , R51H3 , RAD51L2

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion

Background

This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Rad51C antibody, N-term used in Western Blot (WB). GTX89889

GTX89889 WB Image

WB analysis of HeLa lysate using GTX89889 Rad51C antibody, N-term.
Dilution : 2μg/ml
Loading : 30μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399