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Rad52 antibody [5H9]

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301
Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

Cat No. GTX70301

Host

Mouse

Clonality

Monoclonal

Clone Name

5H9

Isotype

IgG1

Application

WB

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa whole cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Rad52 recombinant protein expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rad52 Homolog, Dna Repair Protein , Rad52

Cellular Localization

Nucleus

Background

DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.

Database

Research Area

DATA IMAGES

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Rad52 antibody [5H9] detects Rad52 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] (GTX70301) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Western blot experiment using Rad52 antibody (GTX70301) to detect human Rad52 protein expression in HeLa whole cell lysate

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329