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Rad52 antibody [5H9]

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301
Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

Cat. No. GTX70301

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H9

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Rad52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa whole cell lysate

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Rad52 recombinant protein expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD52 homolog, DNA repair protein

Cellular Localization

Nucleus

Background

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Western blot experiment using Rad52 antibody (GTX70301) to detect human Rad52 protein expression in HeLa whole cell lysate

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] (GTX70301) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Momcilovic O et al. PLoS One 2010; 5 (10) : e13410 DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.
Application : WB
Reactivity : Human
de Souza-Pinto NC et al. Mol Cell Biol 2009; 29 (16) : 4441-54 The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells.
Reactivity : Human
Crosby ME et al. Cancer Res 2009; 69 (3) : 1221-9 MicroRNA regulation of DNA repair gene expression in hypoxic stress.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations