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Rad52 antibody

Orthogonal
Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884
Anti-Rad52 antibody used in Western Blot (WB). GTX00884
Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884
Anti-Rad52 antibody used in Immunoprecipitation (IP). GTX00884
Anti-Rad52 antibody used in ELISA (ELISA). GTX00884
Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884
Anti-Rad52 antibody used in Western Blot (WB). GTX00884

Cat. No. GTX00884

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat, Hamster
Package
50 μg ($399)
See all Rad52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:1000
IP 1:50-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20mM Sodium Phosphate, 150mM NaCl, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-size recombinant human Rad52 protein, no tag-peptide attached

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD52 homolog, DNA repair protein , RAD52

Cellular Localization

Nucleus

Background

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884

GTX00884 ICC/IF Image

Orthogonal

ICC/IF analysis of U2OS 40-2-6 cells treated with or without 2 mM hydroxyurea (HU) for 24 hrs using GTX00884 Rad52 antibody. The cells were pre-extracted with 0.1% Triton X-100 in PBS, fixed with 4% PFA in PBS. Triton X-100 treatment extracts chromatin-free Rad52 from nuclei.
Scale bars : 20 μm
Dilution : 1:50

Anti-Rad52 antibody used in Western Blot (WB). GTX00884

GTX00884 WB Image

WB analysis of various samples using GTX00884 Rad52 antibody.
Lane 1 : Recombinant human Rad52 protein (0.2 ng)
Lane 2 : HeLa whole cell lysate (17 μg)
Lane 3 : MCF-7 whole cell lysate (27 μg)
Lane 4 : NIH-3T3 whole cell lysate (20 μg)
Lane 5 : CHO whole cell lysate (21 μg)
Dilution : 1:1000

Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884

GTX00884 ICC/IF Image

ICC/IF analysis of HeLa (Upper) and MCF-7 (Lower) cells using GTX00884 Rad52 antibody.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Dilution : 1:1000

Anti-Rad52 antibody used in Immunoprecipitation (IP). GTX00884

GTX00884 IP Image

IP analysis of MCF-7 whole cell lysate using GTX00884 Rad52 antibody.
Lane 1: The eluate
Lane 2: The supernatant
Lane 3: Input (16 μg)
IP antibody : 10 μg
Protien amount: 300 μg in PBS

Anti-Rad52 antibody used in ELISA (ELISA). GTX00884

GTX00884 ELISA Image

ELISA analysis of recombinant human Rad52 protein using GTX00884 Rad52 antibody.
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

Anti-Rad52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00884

GTX00884 ICC/IF Image

ICC/IF analysis of GM637 cells using GTX00884 Rad52 antibody. Photographed with confocal microscope.
Fixation : 4% PFA in PBS, 10 mins
Permeabilization : 1% SDS and 0.5% Triton X-100, 10 mins
Dilution : 1:1000

Anti-Rad52 antibody used in Western Blot (WB). GTX00884

GTX00884 WB Image

WB analysis of various samples using GTX00884 Rad52 antibody.
Lane 1 : MCF-7 whole cell lysate (16 μg)
Lane 2 : PC-12 whole cell lysate (40 μg)
Dilution : 1:1000
SDS-PAGE : 12.5%

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399