WB analysis of various samples using GTX00884 Rad52 antibody.
Lane 1 : MCF-7 whole cell lysate (16 μg)
Lane 2 : PC-12 whole cell lysate (40 μg)
Dilution : 1:1000
SDS-PAGE : 12.5%

ICC/IF analysis of GM637 cells using GTX00884 Rad52 antibody. Photographed with confocal microscope.
Fixation : 4% PFA in PBS, 10 mins
Permeabilization : 1% SDS and 0.5% Triton X-100, 10 mins
Dilution : 1:1000

ELISA analysis of recombinant human Rad52 protein using GTX00884 Rad52 antibody.
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

IP analysis of MCF-7 whole cell lysate using GTX00884 Rad52 antibody.
Lane 1: The eluate
Lane 2: The supernatant
Lane 3: Input (16 μg)
IP antibody : 10 μg
Protien amount: 300 μg in PBS

ICC/IF analysis of HeLa (Upper) and MCF-7 (Lower) cells using GTX00884 Rad52 antibody.
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Dilution : 1:1000

WB analysis of various samples using GTX00884 Rad52 antibody.
Lane 1 : Recombinant human Rad52 protein (0.2 ng)
Lane 2 : HeLa whole cell lysate (17 μg)
Lane 3 : MCF-7 whole cell lysate (27 μg)
Lane 4 : NIH-3T3 whole cell lysate (20 μg)
Lane 5 : CHO whole cell lysate (21 μg)
Dilution : 1:1000

ICC/IF analysis of U2OS 40-2-6 cells treated with or without 2 mM hydroxyurea (HU) for 24 hrs using GTX00884 Rad52 antibody. The cells were pre-extracted with 0.1% Triton X-100 in PBS, fixed with 4% PFA in PBS. Triton X-100 treatment extracts chromatin-free Rad52 from nuclei.
Scale bars : 20 μm
Dilution : 1:50