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Rad9 antibody

Anti-Rad9 antibody used in Western Blot (WB). GTX100078
Anti-Rad9 antibody used in IHC (Paraffin sections) (IHC-P). GTX100078
Anti-Rad9 antibody used in Western Blot (WB). GTX100078
Anti-Rad9 antibody used in Western Blot (WB). GTX100078
Anti-Rad9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100078

Cat. No. GTX100078

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rad9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD9 checkpoint clamp component A , RAD9

Cellular Localization

Nucleus

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:1000

Anti-Rad9 antibody used in IHC (Paraffin sections) (IHC-P). GTX100078

GTX100078 IHC-P Image

Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (GTX100078) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:500

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:500

Anti-Rad9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100078

GTX100078 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (GTX100078) antibody at 1:100 dilution.

REFERENCE

Application Reference

Chen KY et al. PLoS One 2019; 14 (7) : e0219317 Resveratrol induced premature senescence and inhibited epithelial-mesenchymal transition of cancer cells via induction of tumor suppressor Rad9.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169