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Rad9 antibody

Anti-Rad9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100078
Anti-Rad9 antibody used in IHC (Paraffin sections) (IHC-P). GTX100078
Anti-Rad9 antibody used in Western Blot (WB). GTX100078
Anti-Rad9 antibody used in Western Blot (WB). GTX100078
Anti-Rad9 antibody used in Western Blot (WB). GTX100078

Cat No. GTX100078

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A431 , HeLa , C2C12 , Rat2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RAD9 checkpoint clamp component A , RAD9

Cellular Localization

Nucleus

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Rad9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100078

GTX100078 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (GTX100078) antibody at 1:100 dilution.

Anti-Rad9 antibody used in IHC (Paraffin sections) (IHC-P). GTX100078

GTX100078 IHC-P Image

Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (GTX100078) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:500

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:1000

Anti-Rad9 antibody used in Western Blot (WB). GTX100078

GTX100078 WB Image

Rad9 antibody detects Rad9 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (GTX100078) dilution: 1:500

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169