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Rad9 (phospho Ser328) antibody

Anti-Rad9 (phospho Ser328) antibody used in Western Blot (WB). GTX50885
Anti-Rad9 (phospho Ser328) antibody used in IHC (Paraffin sections) (IHC-P). GTX50885
Anti-Rad9 (phospho Ser328) antibody used in Dot blot (Dot). GTX50885

Cat. No. GTX50885

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, Dot

Reactivity

 Human
Package
400 μl ($399)
See all Rad9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
Dot 1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S328 of human RAD9.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD9 checkpoint clamp component A , RAD9

Cellular Localization

Nucleus

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Rad9 (phospho Ser328) antibody used in Western Blot (WB). GTX50885

GTX50885 WB Image

WB analysis of HL-60 and Y79 cell lysate using GTX50885 Rad9 (phospho Ser328) antibody.

Anti-Rad9 (phospho Ser328) antibody used in IHC (Paraffin sections) (IHC-P). GTX50885

GTX50885 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX50885 Rad9 (phospho Ser328) antibody.

Anti-Rad9 (phospho Ser328) antibody used in Dot blot (Dot). GTX50885

GTX50885 Dot Image

Dot Blot analysis of Rad9 phospho- or Non phospho-peptide using GTX50885 Rad9 (phospho Ser328) antibody. 50ng of Phospho-peptide or Non phospho-peptide per dot were adsorbed.
Dilution : 0.5μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399