Radixin antibody [N3C3]

Name: Radixin antibody [N3C3]
Brand: GeneTex
SKU: GTX114219
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Radixin antibody [N3C3] used in Western Blot (WB). GTX114219

Anti-Radixin antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114219

Cat. No. GTX114219

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all Radixin products

Datasheet
Document

View product citations for antibody GTX114219 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

HepG2 , 293T

Predict Reactivity

Mouse, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Radixin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

radixin , DFNB24

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton

Background

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. [provided by RefSeq]

Database

Gene ID: 5962 RDX
UniProt: P35241 RDX

Research Area

DATA IMAGES

GTX114219 WB Image

Wild-type (WT) and Radixin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Radixin antibody [N3C3] (GTX114219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114219 IHC-P Image

Radixin antibody [N3C3] detects Radixin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Radixin antibody [N3C3] (GTX114219) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114219 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX114219 diluted at 1:1000

REFERENCE

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REVIEW