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Radixin antibody

KO/KD
Anti-Radixin antibody used in Western Blot (WB). GTX105408
Anti-Radixin antibody used in IHC (Paraffin sections) (IHC-P). GTX105408
Anti-Radixin antibody used in Western Blot (WB). GTX105408
Anti-Radixin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105408
Anti-Radixin antibody used in Western Blot (WB). GTX105408
Anti-Radixin antibody used in IHC (Paraffin sections) (IHC-P). GTX105408
Anti-Radixin antibody used in Western Blot (WB). GTX105408

Cat. No. GTX105408

Reference 14
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Radixin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

mouse liver , 293T

Product Note

Based on sequence homology, this antibody may cross with human MSN and EZR protein.

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Radixin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951675

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

radixin , DFNB24

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton

Background

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Radixin antibody used in Western Blot (WB). GTX105408

GTX105408 WB Image

KO/KD

Wild-type (WT) and Radixin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Radixin antibody (GTX105408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Radixin antibody used in IHC (Paraffin sections) (IHC-P). GTX105408

GTX105408 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Radixin antibody used in Western Blot (WB). GTX105408

GTX105408 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX105408 diluted at 1:3000

Anti-Radixin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105408

GTX105408 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Radixin(GTX105408) antibody at 1:200 dilution.

Anti-Radixin antibody used in Western Blot (WB). GTX105408

GTX105408 WB Image

Radixin antibody detects RDX protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Radixin antibody (GTX105408) dilution: 1:1000

Anti-Radixin antibody used in IHC (Paraffin sections) (IHC-P). GTX105408

GTX105408 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Radixin antibody used in Western Blot (WB). GTX105408

GTX105408 WB Image

The data was published in the journal Breast Cancer Res in 2014. PMID: 24512624

REFERENCE

Application Reference

Rina Doukuni et al. J Clin Med 2022; 11 (13) : 3830 Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells
Application : WB, ICC/IF
Reactivity : Human
Mayuka Tameishi et al. J Clin Med 2022; 11 (9) : 2457 Ezrin Contributes to the Plasma Membrane Expression of PD-L1 in A2780 Cells
Application : WB, ICC/IF
Reactivity : Human
Chihiro Tanaka et al. J Clin Med 2022; 11 (8) : 2226 Ezrin Regulates the Cell Surface Localization of PD-L1 in HEC-151 Cells
Application : WB, ICC/IF
Reactivity : Human
Kobori T. et al. Immuno 2022; 2 (1) : 68-84 Ezrin and Radixin Differentially Modulate Cell Surface Expression of Programmed Death Ligand-1 in Human Pancreatic Ductal Adenocarcinoma KP-2 Cells.
Application : WB, ICC/IF
Reactivity : Human
Chihiro Tanaka et al. Molecules 2021; 26 (18) Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells.
Application : WB, ICC/IF, IP
Reactivity : Human
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Submit a Reference

REVIEW

Radixin antibody Cat. No. GTX105408

Rating
( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Anti-Radixin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105408
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : LS180 cells
Blocking : 1% BSA, 10% Normal Goat Serum, 0.3 M Glycine, 0.1% Tween 20/D-PBS, 25°C, 1Hr
Fixation : 4% PFA, 25°C, 1Hr
Permeabilization /Antigen retrieval : 1% BSA, 10% Normal Goat Serum, 0.3 M Glycine, 0.1% Tween 20/D-PBS
Primary Antibody : 1:50, 4°C, 16Hr
Figure Note : GTX105408 detects radixin protein at plasma membrane and cytosol by immunofluorescent analysis.
Red: Radixin stained by GTX105408
Blue: Fluoroshield with DAPI.
Scale bars represent 20 μm.
Submit a Review
Application Reference
Rina Doukuni et al. J Clin Med 2022; 11 (13):3830 Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells
Application : WB, ICC/IF
Reactivity : Human
Mayuka Tameishi et al. J Clin Med 2022; 11 (9):2457 Ezrin Contributes to the Plasma Membrane Expression of PD-L1 in A2780 Cells
Application : WB, ICC/IF
Reactivity : Human
Chihiro Tanaka et al. J Clin Med 2022; 11 (8):2226 Ezrin Regulates the Cell Surface Localization of PD-L1 in HEC-151 Cells
Application : WB, ICC/IF
Reactivity : Human
Kobori T. et al. Immuno 2022; 2 (1):68-84 Ezrin and Radixin Differentially Modulate Cell Surface Expression of Programmed Death Ligand-1 in Human Pancreatic Ductal Adenocarcinoma KP-2 Cells.
Application : WB, ICC/IF
Reactivity : Human
Chihiro Tanaka et al. Molecules 2021; 26 (18) Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells.
Application : WB, ICC/IF, IP
Reactivity : Human
Tameishi M et al. Pharmaceuticals (Basel) 2021; 14 (10) Contribution of Ezrin on the Cell Surface Plasma Membrane Localization of Programmed Cell Death Ligand-1 in Human Choriocarcinoma JEG-3 Cells.
Application : ICC/IF
Reactivity : Human
Kobori T et al. Pharmaceuticals (Basel) 2021; 14 (9) Role of Ezrin/Radixin/Moesin in the Surface Localization of Programmed Cell Death Ligand-1 in Human Colon Adenocarcinoma LS180 Cells.
Application : WB
Reactivity : Human
Tameishi M. et al. Pharmaceuticals 2021; 14 (10):963 Contribution of Ezrin on the Cell Surface Plasma Membrane Localization of Programmed Cell Death Ligand-1 in Human Choriocarcinoma JEG-3 Cells.
Application : ICC/IF
Reactivity : Human
Tanaka C. et al. Molecules 2021; 26 (18):5648 Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells.
Application : WB, ICC/IF, IP
Reactivity : Human
Kobori T et al. PLoS One 2021; 16 (5):e0250889 Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.
Application : ICC/IF
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Ogiwara K et al. Biol Reprod 2016; A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells.
Application : WB
Babina IS et al. Breast Cancer Res 2014; 16 (1):R19 A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44.
Application : WB
Reactivity : Human
Beaty BT et al. J Cell Biol 2014; (Epub) Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis.
Application : ICC/IF
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169