Wild-type (WT) and Radixin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Radixin antibody (GTX105408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX105408 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Radixin antibody detects RDX protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Radixin antibody (GTX105408) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Radixin(GTX105408) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Breast Cancer Res in 2014. PMID: 24512624