GeneTex
United States (US)

Radixin antibody

GTX105408_39939_20180316_WB_KO_watermark_19011506_844.jpg
GTX105408_39939_IHC_R_19011506_751.jpg
GTX105408_39939_WB_M_liver_19011506_959.jpg
GTX105408_39939_IHC_19011506_592.jpg
GTX105408_IFA_19011506_117.jpg

Cat No. GTX105408

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , mouse liver

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Radixin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Radixin,Dfnb24,Rdx

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton

Background

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105408_39939_20180316_WB_KO_watermark_19011506_844.jpg

GTX105408 WB Image

Wild-type (WT) and Radixin knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Radixin antibody (GTX105408) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX105408_39939_IHC_R_19011506_751.jpg

GTX105408 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105408_39939_WB_M_liver_19011506_959.jpg

GTX105408 WB Image

Radixin antibody detects RDX protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
7.5 % SDS-PAGE
Radixin antibody (GTX105408) dilution: 1:1000

GTX105408_39939_IHC_19011506_592.jpg

GTX105408 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Radixin(GTX105408) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105408_IFA_19011506_117.jpg

GTX105408 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Radixin(GTX105408) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf