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Raf1 antibody [410]

Cat. No. GTX20656

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 410

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Raf1 products

APPLICATION

Application Note

For WB: A concentration of 1.0 μg/ml, will allow visualization of 0.2 μg/lane of human Raf-1. For ELISA: A concentration of 1.0 μg/ml, will allow visualization of 0.2 μg/lane of human Raf-1. Optimal dilutions/concentrations should be determined by the end user

Calculated MW

73 kDa. ( Note )

Product Note

This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Raf-1 (mw:75 kDa).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Cellular Localization

Cytoplasm , Cell membrane , Mitochondrion , Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations