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Raf1 antibody [4G4]

Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60561
Anti-Raf1 antibody [4G4] used in ELISA (ELISA). GTX60561
Anti-Raf1 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX60561
Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60561
Anti-Raf1 antibody [4G4] used in Flow cytometry (FACS). GTX60561

Cat. No. GTX60561

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Raf1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human RAF1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Cellular Localization

Cytoplasm,Cell membrane,Mitochondrion,Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60561

GTX60561 WB Image

WB analysis of HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate using GTX60561 Raf1 antibody [4G4].

Anti-Raf1 antibody [4G4] used in ELISA (ELISA). GTX60561

GTX60561 ELISA Image

ELISA analysis of antigen using GTX60561 Raf1 antibody [4G4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Raf1 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX60561

GTX60561 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60561 Raf1 antibody [4G4].

Anti-Raf1 antibody [4G4] used in Western Blot (WB). GTX60561

GTX60561 WB Image

WB analysis of human RAF1 (AA: 198-407) recombinant protein using GTX60561 Raf1 antibody [4G4].

Anti-Raf1 antibody [4G4] used in Flow cytometry (FACS). GTX60561

GTX60561 FACS Image

FACS analysis of MCF-7 cells using GTX60561 Raf1 antibody [4G4].
Green : Raf1
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409