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Raf1 antibody [N1C1-2]

Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588
Anti-Raf1 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111588
Anti-Raf1 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX111588
Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588

Cat No. GTX111588

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951612

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588

GTX111588 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N1C1-2] (GTX111588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raf1 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111588

GTX111588 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Raf1(GTX111588) antibody at 1:200 dilution.

Anti-Raf1 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX111588

GTX111588 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Raf1(GTX111588) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Raf1 antibody [N1C1-2] used in Western Blot (WB). GTX111588

GTX111588 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N1C1-2] (GTX111588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169