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Raf1 antibody [N3C3]

Orthogonal Overexpression
Anti-Raf1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107763
Anti-Raf1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107763
Anti-Raf1 antibody [N3C3] used in Western Blot (WB). GTX107763
Anti-Raf1 antibody [N3C3] used in Western Blot (WB). GTX107763
Anti-Raf1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107763

Cat. No. GTX107763

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Raf1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

RAF1-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Raf1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107763

GTX107763 IHC-P Image

Raf1 antibody [N3C3] detects Raf1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Raf1 stained by Raf1 antibody [N3C3] (GTX107763) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Raf1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107763

GTX107763 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Raf1(GTX107763) antibody at 1:200 dilution.

Anti-Raf1 antibody [N3C3] used in Western Blot (WB). GTX107763

GTX107763 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raf1 antibody [N3C3] used in Western Blot (WB). GTX107763

GTX107763 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raf1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107763

GTX107763 IHC-P Image

Raf1 antibody [N3C3] detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Raf1 antibody [N3C3] (GTX107763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.
Wang TH et al. Int J Mol Sci 2018; 19 (9) Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Application : WB
Reactivity : Human
Lin JT et al. Journal of Functional Foods 2018; 47 : 343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Lappas M et al. Reproduction 2016; 151 (4) : 411-20 RAF1 is increased in labouring myometrium and modulates inflammation-induced pro-labour mediators.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169