GeneTex
United States (US)

Raf1 antibody [N3C3]

GTX107763_42907_20181109_WB_B_19011519_998.jpg
GTX107763_43110_20180928_IHC-P_19011507_709.jpg
GTX107763_40016_IHC_M_19011507_498.jpg
GTX107763_43110_20180202_WB_treatment_PMA_19011507_251.jpg
GTX107763_IFA_19011507_676.jpg

Cat No. GTX107763

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

HeLa (starvation for 24 hr) , HeLa (starvation for 24 hr and 200 nM PMA treatment for 30 min)

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Raf-1 Proto-Oncogene, Serine/Threonine Kinase,Cmd1Nn,Craf,Ns5,Raf-1,C-Raf,Raf1

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107763_42907_20181109_WB_B_19011519_998.jpg

GTX107763 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107763_43110_20180928_IHC-P_19011507_709.jpg

GTX107763 IHC-P Image

Raf1 antibody [N3C3] detects Raf1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Raf1 stained by Raf1 antibody [N3C3] (GTX107763) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107763_40016_IHC_M_19011507_498.jpg

GTX107763 IHC-P Image

Raf1 antibody [N3C3] detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Raf1 antibody [N3C3] (GTX107763) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107763_43110_20180202_WB_treatment_PMA_19011507_251.jpg

GTX107763 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N3C3] (GTX107763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107763_IFA_19011507_676.jpg

GTX107763 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Raf1(GTX107763) antibody at 1:200 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf