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Raf1 (phospho Ser259) antibody [GT1309]

Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221
Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221
Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221

Cat No. GTX03221

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1309

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S259 of human Raf1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Cellular Localization

Cytoplasm , Cell membrane , Mitochondrion , Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221

GTX03221 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 (phospho Ser259) antibody [GT1309] (GTX03221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221

GTX03221 WB Image

WB analysis of 293T cells were treated by Forskolin (10 uM) at 37ºC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309].
Dilution : 1:1000
Loading : 25μg per lane

Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221

GTX03221 WB Image

WB analysis of C6 cells were treated by Forskolin (10 uM) at 37ºC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for Raf1 (phospho Ser259) antibody [GT1309] (GTX03221). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Raf1 (phospho Ser259) antibody [GT1309] (GTX03221). Be the first to share your experience with this product.
Package List Price ($)
$ 379