APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.25 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding S259 of human Raf1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf
Cellular Localization
Cytoplasm , Cell membrane , Mitochondrion , Nucleus
Background
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/templates/default/js/lightbox/images/loading.gif "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
|
GTX03221 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 (phospho Ser259) antibody [GT1309] (GTX03221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX03221 WB Image
WB analysis of C6 cells were treated by Forskolin (10 uM) at 37ºC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309].
Dilution : 1:1000
Loading : 25μg per lane
|
|
|
GTX03221 WB Image
WB analysis of 293T cells were treated by Forskolin (10 uM) at 37ºC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309].
Dilution : 1:1000
Loading : 25μg per lane
|
REFERENCE
There are currently no references for Raf1 (phospho Ser259) antibody [GT1309] (GTX03221). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Raf1 (phospho Ser259) antibody [GT1309] (GTX03221). Be the first to share your experience with this product.
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/small/GTX03221/GTX03221_4000001557_20210716_WB_CIP_w_23053123_118.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/small/GTX03221/GTX03221_81_WB_w_23053123_624.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/small/GTX03221/GTX03221_80_WB_w_23053123_762.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/normal/GTX03221/GTX03221_4000001557_20210716_WB_CIP_w_23053123_118.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/normal/GTX03221/GTX03221_81_WB_w_23053123_624.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")
![Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221](/upload/website/prouct_img/normal/GTX03221/GTX03221_80_WB_w_23053123_762.webp "Anti-Raf1 (phospho Ser259) antibody [GT1309] used in Western Blot (WB). GTX03221")