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Raf1 (phospho Ser259) antibody

Anti-Raf1 (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX78955
Anti-Raf1 (phospho Ser259) antibody used in Western Blot (WB). GTX78955

Cat No. GTX78955

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-Sp-T-P).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11164862

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CMD1NN,CRAF,NS5,RAF1,Raf1,Raf1 protooncogene, serine/threonine kinase,cRaf,c-Raf,Raf-1 proto-oncogene, serine/threonine kinase

Cellular Localization

Cytoplasm,Cell membrane,Mitochondrion,Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Raf1 (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX78955

GTX78955 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78955 Raf1 (phospho Ser259) antibody.

Anti-Raf1 (phospho Ser259) antibody used in Western Blot (WB). GTX78955

GTX78955 WB Image

WB analysis of K562 and MDA-MB-435 cell lysates using GTX78955 Raf1 (phospho Ser259) antibody (Lane 4, 5, and 6) and Raf1 antibody (Lane 1, 2, and 3).

REFERENCE

REVIEW

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Package List Price ($)
$ 339