GeneTex
United States (US)

Raf1 (phospho Ser43) antibody

GTX30714_4_Dot_18121410_224.jpg

Cat No. GTX30714

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Dot
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Dot 1:500
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S43 of human RAF1.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Raf-1 Proto-Oncogene, Serine/Threonine Kinase,Cmd1Nn,Craf,Ns5,Raf-1,C-Raf,Raf1

Background

The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated phosphorylates MEK which in turn phosphorylates ERK. Serine 43 of c-Raf is phosphorylated by PKA, and is thought to negatively affect the ability of Ras to activate c-Raf.

Database

Research Area

DATA IMAGES
GTX30714_4_Dot_18121410_224.jpg

GTX30714 Dot Image

Dot Blot analysis of Raf1 phospho- or Non phospho-peptide using GTX30714 Raf1 (phospho Ser43) antibody. 50ng of Phospho-peptide or Non phospho-peptide per dot were adsorbed.
Dilution : 0.5μg/ml