ICC/IF analysis of U2OS cells using GTX65906 Raf1 (phospho Ser621) antibody.
Blue : DAPI

WB analysis of HeLa cells treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight using GTX65906 Raf1 (phospho Ser621) antibody.
Dilution : 1:1000
Loading : 25μg per lane

IP analysis of HeLa cell lysate using GTX65906 Raf1 (phospho Ser621) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000