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Raf1 (phospho Ser621) antibody

Anti-Raf1 (phospho Ser621) antibody used in Immunoprecipitation (IP). GTX65906
Anti-Raf1 (phospho Ser621) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65906
Anti-Raf1 (phospho Ser621) antibody used in Western Blot (WB). GTX65906

Cat No. GTX65906

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S621 of human RAF1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Cellular Localization

Cytoplasm,Cell membrane,Mitochondrion,Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Raf1 (phospho Ser621) antibody used in Immunoprecipitation (IP). GTX65906

GTX65906 IP Image

IP analysis of HeLa cell lysate using GTX65906 Raf1 (phospho Ser621) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-Raf1 (phospho Ser621) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65906

GTX65906 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX65906 Raf1 (phospho Ser621) antibody.
Blue : DAPI

Anti-Raf1 (phospho Ser621) antibody used in Western Blot (WB). GTX65906

GTX65906 WB Image

WB analysis of HeLa cells treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight using GTX65906 Raf1 (phospho Ser621) antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for Raf1 (phospho Ser621) antibody (GTX65906). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339