Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/1,000 |
Note :
WB
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result i
|
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Mouse, Bovine, Dog, Chicken(>80% identity)
Form
Liquid
Buffer
10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
Raf-1 proto-oncogene, serine/threonine kinase
Cellular Localization
Cytoplasm , Cell membrane , Mitochondrion , Nucleus
Background
acts as a mitogenic protein kinase; mutant forms may play a role in transformation [RGD, Feb 2006]
Database
Research Area