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Raf1 (phospho Tyr340/Tyr341) antibody

Anti-Raf1 (phospho Tyr340/Tyr341) antibody used in Western Blot (WB). GTX24769

Cat No. GTX24769

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse, Rat
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antibody was produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains tyrosines 340 and 341. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Raf-1 proto-oncogene, serine/threonine kinase , CMD1NN , CRAF , NS5 , Raf-1 , c-Raf

Cellular Localization

Cytoplasm,Cell membrane,Mitochondrion,Nucleus

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Raf1 (phospho Tyr340/Tyr341) antibody used in Western Blot (WB). GTX24769

GTX24769 WB Image

WB (peptide competition) analysis of HEK293 cells overexpressing c-Raf unstimulated (Lane 1) or stimulated with active Src (Lane 2-5) using GTX24769 Raf1 (phospho Tyr340/Tyr341) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319