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Rag C antibody

Anti-Rag C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00731
Anti-Rag C antibody used in Western Blot (WB). GTX00731

Cat. No. GTX00731

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($399)
See all Rag C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified full-length human Rag C protein fused with GST

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras related GTP binding C , GTR2 , RAGC , TIB929

Cellular Localization

Cytoplasm,Nucleus,Lysosome

Background

This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-Rag C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00731

GTX00731 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00731 Rag C antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:100
Fixation : 4% PFA
Permeabilization : 0.5% Triton X-100

Anti-Rag C antibody used in Western Blot (WB). GTX00731

GTX00731 WB Image

WB analysis of various samples using GTX00731 Rag C antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : MOLT-4 whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Lane 5 : CHO whole cell lysate
Dilution : 1:1000
Loading : 20μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399