GeneTex
United States (US)

RanBP1 antibody, C-term

GTX17034_1509_WB_18121410_753.jpg
GTX17034_1074_IHC-P_18121410_310.jpg

Cat No. GTX17034

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.05-0.1μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Kidney shows pixulated cytoplasm staining in DCT.

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17034-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-LSVKEETKEDAEEKQ, from the C Terminus of the protein sequence according to NP_002873.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ran Binding Protein 1,Htf9A,Ranbp1

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17034_1509_WB_18121410_753.jpg

GTX17034 WB Image

WB analysis of HeLa lysate using GTX17034 RanBP1 antibody, C-term.
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer

GTX17034_1074_IHC-P_18121410_310.jpg

GTX17034 IHC-P Image

IHC-P analysis of human kidney using GTX17034 RanBP1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329