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RanBP1 antibody [HL2358]

Recombinant antibody KO/KD
Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564
Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564
Anti-RanBP1 antibody [HL2358] used in IHC (Paraffin sections) (IHC-P). GTX638564
Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564
Anti-RanBP1 antibody [HL2358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638564

Cat. No. GTX638564

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2358

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RanBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human RanBP1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAN binding protein 1 , HTF9A

Background

This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564

GTX638564 WB Image

KO/KD

Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (GTX638564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564

GTX638564 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (GTX638564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP1 antibody [HL2358] used in IHC (Paraffin sections) (IHC-P). GTX638564

GTX638564 IHC-P Image

RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RanBP1 stained by RanBP1 antibody [HL2358] (GTX638564) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RanBP1 antibody [HL2358] used in Western Blot (WB). GTX638564

GTX638564 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (GTX638564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP1 antibody [HL2358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638564

GTX638564 ICC/IF Image

RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RanBP1 stained by RanBP1 antibody [HL2358] (GTX638564) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations