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RanBP1 antibody [N1C3]

KO/KD
Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192
Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192
Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192
Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192
Anti-RanBP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103192

Cat. No. GTX103192

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RanBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse brain , HeLaS3 , 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RanBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951628

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAN binding protein 1 , HTF9A

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192

GTX103192 WB Image

KO/KD

Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192

GTX103192 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [N1C3] (GTX103192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192

GTX103192 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa S3 (GTX14654)
12% SDS PAGE
GTX103192 diluted at 1:1000

Anti-RanBP1 antibody [N1C3] used in Western Blot (WB). GTX103192

GTX103192 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX103192 diluted at 1:1000

Anti-RanBP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103192

GTX103192 ICC/IF Image

Ran BP1 antibody [N1C3] detects Ran BP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ran BP1 protein stained by Ran BP1 antibody [N1C3] (GTX103192) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Chao HW et al. Nucleic Acids Res 2012; 40 (17) : 8484-98 NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169