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RanBP16 antibody [HL2510]

Recombinant antibody KO/KD
Anti-RanBP16 antibody [HL2510] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638871
Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871
Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871
Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871

Cat. No. GTX638871

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2510

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RanBP16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human RanBP16.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exportin 7 , EXP7 , RANBP16

Cellular Localization

Cytoplasm,Nucleus

Background

The transport of protein and large RNAs through the nuclear pore complexes (NPC) is an energy-dependent and regulated process. The import of proteins with a nuclear localization signal (NLS) is accomplished by recognition of one or more clusters of basic amino acids by the importin-alpha/beta complex; see MIM 600685 and MIM 602738. The small GTPase RAN (MIM 601179) plays a key role in NLS-dependent protein import. RAN-binding protein-16 is a member of the importin-beta superfamily of nuclear transport receptors.[supplied by OMIM, Jul 2002]

Database

Research Area

DATA IMAGES

Anti-RanBP16 antibody [HL2510] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638871

GTX638871 ICC/IF Image

RanBP16 antibody [HL2510] detects RanBP16 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA.
Green: RanBP16 stained by RanBP16 antibody [HL2510] (GTX638871) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871

GTX638871 WB Image

KO/KD

Non-transfected (–) and transfected (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (GTX638871) diluted at 1:9000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871

GTX638871 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (GTX638871) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RanBP16 antibody [HL2510] used in Western Blot (WB). GTX638871

GTX638871 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (GTX638871) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations