APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:2000 |
1:200-1:1000 |
1:200 |
Assay dependent |
Note :
IHC-P
Recommend using a heat mediated antigen retrieval with citrate buffer (pH 6.0).
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Recombinant human RanBP9 from Phe133 to Tyr 229
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAN binding protein 9 , BPM-L , BPM90 , RANBPM , RanBP7
Cellular Localization
Cytoplasm,Nucleus,Cell membrane
Background
This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00728 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00728 RanBP9 antibody. Dilution : 1:1000 Fixation : 4% PFA
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GTX00728 WB Image
WB analysis of various samples using GTX00728 RanBP9 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : KB whole cell lysate Lane 3 : Cos-7 whole cell lysate Lane 4 : BHK21 whole cell lysate Lane 5 : CHO whole cell lysate Lane 6 : WEHI whole cell lysate Lane 7 : FM3A whole cell lysate Dilution : 1:1000 Loading : 50 μg
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GTX00728 WB Image
WB analysis of various samples using GTX00728 RanBP9 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : U2OS whole cell lysate Dilution : 1:2000
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REFERENCE
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REVIEW
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