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RanGAP1 antibody, N-term

Anti-RanGAP1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX22081
Anti-RanGAP1 antibody, N-term used in Western Blot (WB). GTX22081

Cat. No. GTX22081

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all RanGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Liver shows vesicular staining in the cytoplasm of hepatocytes.
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22081-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence ASEDIAKLAETLAK-C, from the N Terminus of the protein sequence according to NP_002874.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ran GTPase activating protein 1 , Fug1 , RANGAP , SD

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-RanGAP1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX22081

GTX22081 IHC-P Image

IHC-P analysis of human liver using GTX22081 RANGAP1 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-RanGAP1 antibody, N-term used in Western Blot (WB). GTX22081

GTX22081 WB Image

WB analysis of 3T3 lysate using GTX22081 RANGAP1 antibody, N-term.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399