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RanGAP1 antibody [N1], N-term

Anti-RanGAP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX106338
Anti-RanGAP1 antibody [N1], N-term used in Western Blot (WB). GTX106338

Cat No. GTX106338

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RanGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241242

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ran GTPase activating protein 1 , Fug1 , RANGAP , SD

Cellular Localization

Cytoplasm , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side , Kinetochore

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RanGAP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX106338

GTX106338 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Trachea, using RanGAP1(GTX106338) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RanGAP1 antibody [N1], N-term used in Western Blot (WB). GTX106338

GTX106338 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106338 diluted at 1:1000

REFERENCE

There are currently no references for RanGAP1 antibody [N1], N-term (GTX106338). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RanGAP1 antibody [N1], N-term (GTX106338). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169